CELL-SHAPE AND CARTILAGE DIFFERENTIATION OF EARLY CHICK LIMB BUD CELLS IN CULTURE
CELL-SHAPE AND CARTILAGE DIFFERENTIATION OF EARLY CHICK LIMB BUD CELLS IN CULTURE
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DOI：
10.1016/0045-6039(82)90072-0
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
WOLPERT, L
中科院分区：
文献类型：
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作者：
ARCHER, CW;ROONEY, P;WOLPERT, L
在胚胎雏鸡的软骨形成开始之前，预期的软骨细胞在软骨形成前凝聚。在培养中，只有四肢中胚层细胞以高于融合密度的方式培养，才能表达混生表型。这导致了一种建议，即软骨分化需要在明显的软骨形成之前立即进行组织起源的相互作用。在体内和体外情况下，分泌软骨基质的细胞呈圆形。成熟软骨细胞的表型表达与细胞形状有关。在半黏附基质(聚甲基丙烯酸羟乙酯)上保持圆形形态的细胞比在正常组织培养塑料上扁平的细胞合成更多含有S的细胞外基质。研究了鸡胚胎中胚成软骨细胞分化为分泌活跃的软骨母细胞是否存在相似的形态依赖关系。圆形的细胞形状有利于硫酸盐化基质的合成。
The onset of chondrogenesis in the embryonic chick is preceded by a prechondrogenic condensation of the prospective cartilage cells. In culture, the condrogenic phenotype was only expressed by limb mesoderm cells plated at densities above confluence. This led to the proposal that condrogenic differentiation requires immediate histogenic interactions prior to overt chondrogenesis. In both the in vivo and in vitro situations, cells secreting a cartilaginous matrix were rounded in shape. A dependence of phenotypic expression by mature chondrocytes on cell shape was shown. Cells maintained in a rounded configuration by culturing on a semiadhesive substratum (poly(HEMA[hydroxy-ethyl-methacrylate])) synthesized more S-containing extracellular matrix than cells allowed to flatten on normal tissue culture plastic did. Whether there is a similar shape-dependent relationship in the differentiation of chick embryonic mesoblasts into actively secreting chondroblasts was investigated. A rounded cell shape was conducive to the synthesis of a sulfated matrix.