Comparison of mesenchymal tissues-derived stem cells for in vivo chondrogenesis: suitable conditions for cell therapy of cartilage defects in rabbit
Comparison of mesenchymal tissues-derived stem cells for in vivo chondrogenesis: suitable conditions for cell therapy of cartilage defects in rabbit
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DOI：
10.1007/s00441-008-0633-5
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发表时间：
2008-08-01
影响因子：
3.6
通讯作者：
Sekiya, Ichiro
中科院分区：
文献类型：
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作者：
Koga, Hideyuki;Muneta, Takeshi;Sekiya, Ichiro
我们之前比较了来自各种间充质组织的间充质干细胞（MSCs），并证明滑膜间充质干细胞在人和大鼠体内具有最佳的体外扩增和成软骨能力。在本研究中，我们比较了兔间充质干细胞的体内成软骨潜能。我们还检查了其他参数，以阐明体外和体内软骨形成的合适条件。从成年兔的骨髓、滑膜、脂肪组织和肌肉中分离出MSCs。比较增殖潜能和体外成软骨潜能。我们还研究了示踪剂DiI对体外软骨形成的毒性。将各组织间充质干细胞包埋于胶原凝胶中，移植到兔全层软骨缺损中。组织学上比较软骨基质的生成。我们还研究了细胞密度和骨膜贴片对滑膜间充质干细胞体内成软骨潜能的影响。滑膜间充质干细胞和肌肉间充质干细胞比其他细胞具有更高的增殖潜能。滑膜和骨髓间充质干细胞颗粒显示丰富的软骨基质。DiI对体外软骨形成无显著影响。移植到软骨缺损后，滑膜间充质干细胞和骨髓间充质干细胞比其他细胞产生更多的软骨基质。当滑膜间充质干细胞以更高的细胞密度和骨膜贴片移植时，观察到更丰富的软骨基质。因此，滑膜和骨髓间充质干细胞比脂肪和肌肉间充质干细胞具有更大的体内成软骨潜能，但滑膜间充质干细胞具有更大的增殖潜能的优势。通过滑膜间充质干细胞获得高产量的软骨基质需要更高的细胞密度和骨膜贴片。
We previously compared mesenchymal stem cells (MSCs) from a variety of mesenchymal tissues and demonstrated that synovium-MSCs had the best expansion and chondrogenic ability in vitro in humans and rats. In this study, we compared the in vivo chondrogenic potential of rabbit MSCs. We also examined other parameters to clarify suitable conditions for in vitro and in vivo cartilage formation. MSCs were isolated from bone marrow, synovium, adipose tissue, and muscle of adult rabbits. Proliferation potential and in vitro chondrogenic potential were compared. Toxicity of the tracer DiI for in vitro chondrogenesis was also examined. MSCs from each tissue were embedded in collagen gel and transplanted into full thickness cartilage defects of rabbits. Cartilage matrix production was compared histologically. The effects of cell density and periosteal patch on the in vivo chondrogenic potential of synovium-MSCs were also examined. Synovium- and muscle-MSCs had a higher proliferation potential than other cells. Pellets from synovium- and bone-marrow-MSCs showed abundant cartilage matrix. DiI had no significant influence on in vitro cartilage formation. After transplantation into cartilage defects, synovium- and bone-marrow-MSCs produced much more cartilage matrix than other cells. When synovium-MSCs were transplanted at a higher cell density and with a periosteal patch, more abundant cartilage matrix was observed. Thus, synovium- and bone-marrow-MSCs had greater in vivo chondrogenic potential than adipose- and muscle-MSCs, but synovium-MSCs had the advantage of a greater proliferation potential. Higher cell density and a periosteum patch were needed to obtain a high production of cartilage matrix by synovium-MSCs.