Temporal and spatial control of neural effects following intracerebral microinfusion.
Temporal and spatial control of neural effects following intracerebral microinfusion.
复制标题
脑内微输注后神经效应的时间和空间控制。
DOI：
10.1080/10611860801886695
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发表时间：
2008
影响因子：
4.5
通讯作者：
Tillery,StephenIHelms
中科院分区：
文献类型：
--
作者：
Stukel,JillM;Parks,Jason;Caplan,MichaelR;Tillery,StephenIHelms
神经药物传递的空间和时间控制对于许多治疗应用和大脑模式和行为的分析至关重要。具体来说，为了观察该部位神经活动的变化，局部注射用于给药或使孤立的组织区域失活，过量分布到周围区域可能会混淆分析或对健康组织产生不利影响。在这里，我们开发了一个质量传递模型，模拟了短时间内初始输注灭活药物，然后用人工脑脊液（aCSF）连续对流洗涤，同时跟踪组织中超过一定阈值浓度的灭活剂区域。我们分析了有效扩散系数、胞外体积分数和注射浓度等参数对时空分布的影响。此外，我们观察了aCSF在初始注射后的冲洗期对注射影响的体积宽度的影响。这些模拟表明，通过注射低浓度的小体积药物，然后用aCSF冲洗，可以在受控的持续时间内改变组织的一个明确描绘的区域。
Spatial and temporal control of neural drug delivery is critical for many therapeutic applications and analyses of brain patterns and behavior. Specifically, for localized injections that serve to deliver drug or inactivate an isolated tissue region in order to observe changes in neural activity at that site, excess distribution into surrounding regions may confound analysis or adversely affect healthy tissue. Here, we develop a mass transport model that simulates a short period of initial infusion of inactivating drug, followed by a successive convective wash with artificial cerebrospinal fluid (aCSF), while tracking the regions of tissue that are above a certain threshold concentration of inactivating agent. We analyze the effect of parameters such as effective diffusion coefficient, extracellular volume fraction, and injectate concentration upon spatiotemporal distribution profiles. Further, we observe the effects of following the initial injection with a wash-out period with aCSF upon the breadth of the volume affected by the injectate. These simulations indicate that, by injecting small volumes of drug at low concentrations and following them with an aCSF flush, a well-delineated region of tissue can be altered for a controlled duration.