The Influence of Electric Fields on Hippocampal Neural Progenitor Cells
The Influence of Electric Fields on Hippocampal Neural Progenitor Cells
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DOI：
10.1007/s12015-010-9171-0
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发表时间：
2010-12-01
影响因子：
4.8
通讯作者：
Mallapragada, Surya K.
中科院分区：
文献类型：
--
作者：
Ariza, Carlos Atico;Fleury, Asha T.;Mallapragada, Surya K.
神经干细胞/祖细胞（npc）的分化和增殖依赖于各种体内环境因素或线索，其中可能包括内源性电场（EF），这是在神经系统发育和修复过程中观察到的。在这项研究中，我们研究了暴露于两种估计内源性强度的电磁场时，成年海马npc的形态、表型和有丝分裂改变。与对照组相比，用垂直于EF向量的437 mV/mm直流电（DC） EF处理的npc更倾向于分化为神经元，而不是少突胶质细胞或星形胶质细胞。此外，在437 mV/mm的直流EF中，NPC过程生长呈垂直促进，阳极抑制。然而，在DC EF中观察到的细胞较少，部分原因是细胞活力降低。施加的另一个EF是46 mV/mm的交流电EF。然而，与控制条件相比，46 mV/mm交流EF在对准或分化方面没有显着差异。对于两种EF处理，实验最后14小时有丝分裂细胞的百分比在统计学上与对照组相似。据我们所知，这是体外EF影响成人鼻咽癌分化的第一个证据。为了阐明电场在控制表型行为中的作用，有必要进一步研究和应用电场在干细胞上的作用。随着研究的进展，利用电磁场可以控制移植细胞的分化和靶向生长，以干细胞为基础的治疗神经系统疾病。
The differentiation and proliferation of neural stem/progenitor cells (NPCs) depend on various in vivo environmental factors or cues, which may include an endogenous electrical field (EF), as observed during nervous system development and repair. In this study, we investigate the morphologic, phenotypic, and mitotic alterations of adult hippocampal NPCs that occur when exposed to two EFs of estimated endogenous strengths. NPCs treated with a 437 mV/mm direct current (DC) EF aligned perpendicularly to the EF vector and had a greater tendency to differentiate into neurons, but not into oligodendrocytes or astrocytes, compared to controls. Furthermore, NPC process growth was promoted perpendicularly and inhibited anodally in the 437 mV/mm DC EF. Yet fewer cells were observed in the DC EF, which in part was due to a decrease in cell viability. The other EF applied was a 46 mV/mm alternating current (AC) EF. However, the 46 mV/mm AC EF showed no major differences in alignment or differentiation, compared to control conditions. For both EF treatments, the percent of mitotic cells during the last 14 h of the experiment were statistically similar to controls. Reported here, to our knowledge, is the first evidence of adult NPC differentiation affected in an EF in vitro. Further investigation and application of EFs on stem cells is warranted to elucidate the utility of EFs to control phenotypic behavior. With progress, the use of EFs may be engineered to control differentiation and target the growth of transplanted cells in a stem cell-based therapy to treat nervous system disorders.