The crystal structure of the TolB box of colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins.
The crystal structure of the TolB box of colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins.
复制标题
DOI：
10.1111/j.1365-2958.2009.06808.x
复制
发表时间：
2010-02
影响因子：
3.6
通讯作者：
James R
中科院分区：
文献类型：
--
作者：
Zhang Y;Li C;Vankemmelbeke MN;Bardelang P;Paoli M;Penfold CN;James R
A组粘菌素的TolB盒与大肠杆菌细胞外周质中的TolB蛋白的相互作用促进了粘菌素的细胞毒性结构域在细胞包膜上的运输。colicin a （ColA）易位域的107个残基肽（TA1-107）与TolB之间的复合物的晶体结构表明，TolB盒子是一个12个残基肽，折叠成一个扭曲的发夹，位于TolB β-螺旋桨结构域的中心峡谷内。将该结构与ColE9 (ColE9) TolB box - TolB复合物的结构进行比较，以及对ColA TolB box残基进行位点定向诱变，揭示了两个TolB box与TolB上重叠结合位点的相互作用的重要差异。用ColE9的TolB盒残基取代ColA的TolB盒残基，使其能够竞争性地从Pal中招募TolB，但降低了突变体ColA的生物活性。这一数据解释了(i) ColA和ColE9与TolB结合亲和力的差异，以及（ii）与ColE9不同，ColA无法竞争性地从Pal中招募TolB，从而可以理解这两种粘菌素如何以不同的方式与大肠杆菌细胞中的共同易位门户相互作用。
Interaction of the TolB box of Group A colicins with the TolB protein in the periplasm of Escherichia coli cells promotes transport of the cytotoxic domain of the colicin across the cell envelope. The crystal structure of a complex between a 107-residue peptide (TA1–107) of the translocation domain of colicin A (ColA) and TolB identified the TolB box as a 12-residue peptide that folded into a distorted hairpin within a central canyon of the β-propeller domain of TolB. Comparison of this structure with that of the colicin E9 (ColE9) TolB box–TolB complex, together with site-directed mutagenesis of the ColA TolB box residues, revealed important differences in the interaction of the two TolB boxes with an overlapping binding site on TolB. Substitution of the TolB box residues of ColA with those of ColE9 conferred the ability to competitively recruit TolB from Pal but reduced the biological activity of the mutant ColA. This datum explains (i) the difference in binding affinities of ColA and ColE9 with TolB, and (ii) the inability of ColA, unlike ColE9, to competitively recruit TolB from Pal, allowing an understanding of how these two colicins interact in a different way with a common translocation portal in E. coli cells.
登录
查看更多内容
DOI：
10.1016/s0969-2126(00)00079-4
发表时间：
2000-01-15
期刊：
STRUCTURE WITH FOLDING & DESIGN
影响因子：
--
作者：
Carr, S;Penfold, CN;Hemmings, AM
通讯作者：
Hemmings, AM
影响因子：
15
作者：
Bonsor, Daniel A.;Grishkovskaya, Irina;Kleanthous, Colin
通讯作者：
Kleanthous, Colin
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
3.6
作者：
Cascales, E;Gavioli, M;Lloubès, R
通讯作者：
Lloubès, R
影响因子：
4.8
作者：
BOUVERET, E;DEROUICHE, R;BENEDETTI, H
通讯作者：
BENEDETTI, H