DNA METHYLATION IN FOLATE-DEFICIENCY - USE OF CPG METHYLASE
DNA METHYLATION IN FOLATE-DEFICIENCY - USE OF CPG METHYLASE
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DOI：
10.1006/bbrc.1993.1750
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发表时间：
1993-06-30
影响因子：
3.1
通讯作者：
WAGNER, C
中科院分区：
文献类型：
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作者：
BALAGHI, M;WAGNER, C
叶酸缺乏导致S-腺苷蛋氨酸/S-腺苷同型半胱氨酸比值明显降低。这个比例调节许多细胞甲基化反应的活性，包括DNA的甲基化反应。我们使用细菌CpG甲基酶来评估叶酸缺乏对断奶大鼠肝脏DNA甲基化的影响，这些大鼠饲喂了氨基酸定义的叶酸缺乏饮食。4周后，与配对喂养的对照动物相比，缺陷动物的肝脏DNA甲基化程度较低。
Folate deficiency produces a marked decrease in the ratio of S-adenosylmethionine to S-adenosylhomocysteine. This ratio regulates the activity of many cellular methylation reactions including that of DNA. We used bacterial CpG methylase to evaluate the effect of folate deficiency on methylation of DNA from livers of weanling rats fed an amino acid-defined folate deficient diet. After 4 weeks, liver DNA from deficient animals was undermethylated compared with DNA from pair-fed control animals.