Rapid selective effects by a growth inhibitor and epidermal growth factor on the incorporation of [35S]methionine into proteins secreted by African green monkey (BSC-1) cells.
Rapid selective effects by a growth inhibitor and epidermal growth factor on the incorporation of [35S]methionine into proteins secreted by African green monkey (BSC-1) cells.
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生长抑制剂和表皮生长因子对 [35S] 蛋氨酸掺入非洲绿猴 (BSC-1) 细胞分泌的蛋白质的快速选择性作用。
DOI：
10.1073/pnas.80.18.5636
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发表时间：
1983
影响因子：
11.1
通讯作者：
Holley,RW
中科院分区：
文献类型：
--
作者：
Nilsen-Hamilton,M;Holley,RW
汇合的非洲绿色猴肾（BSC-1）细胞分泌抑制相同细胞的DNA合成和生长的蛋白质（Mr约等于24，000）。使用[35 S]蛋氨酸代谢标记蛋白质，我们发现这种生长抑制剂选择性地诱导BSC-1细胞合成和分泌另一种蛋白质，其在NaDodSO 4/聚丙烯酰胺凝胶上的相对Mr为48，000。我们将这种蛋白质称为“细胞诱导蛋白”（IIP 48）。由于用生长抑制剂处理，IIP 48标记率的最大增加平均为对照的12倍。IIP 48是一种N-糖苷键连接的糖蛋白，它不是一种主要的细胞内蛋白。在向细胞中加入生长抑制剂的4至6小时内，该蛋白质被最大程度地诱导。这是这些细胞对生长抑制剂的早期反应，可能代表对生长抑制剂的主要反应。表皮生长因子（EGF）增加了其他三种分泌蛋白（MrS 28，000，59，000和61，000）的标记率，我们称之为“促分裂原诱导蛋白”（MIP 28，MIP 59和MIP 61）。如果放线菌素D与生长效应物一起加入，EGF和生长抑制剂对这些蛋白质的分泌水平的特异性作用被抑制。因此，RNA合成似乎是诱导所必需的。EGF和生长抑制剂通过独立和非相互作用的途径诱导这些分泌蛋白。
Confluent African green monkey kidney (BSC-1) cells secrete a protein (Mr approximately equal to 24,000) that inhibits DNA synthesis and growth of the same cells. Using [35S]methionine to metabolically label proteins, we have found that this growth inhibitor selectively induces the BSC-1 cells to synthesize and secrete another protein with a relative Mr of 48,000 on NaDodSO4/polyacrylamide gels. We have called this protein "inhibitor-inducible protein" (IIP48). The maximal increase in rate of labeling of IIP48 due to treatment with the growth inhibitor averages 12-fold over the control. IIP48 is an N-glycosidically linked glycoprotein, and it is not a major intracellular protein. This protein is maximally induced within 4 to 6 hr of adding the growth inhibitor to the cells. This is an early response of these cells to the growth inhibitor and may represent a primary response to the growth inhibitor. Epidermal growth factor (EGF) increases the rate of labeling of three other secreted proteins (MrS 28,000, 59,000, and 61,000), which we have called "mitogen-inducible proteins" (MIP28, MIP59, and MIP61). The specific effects of both EGF and the growth inhibitor on the secreted levels of these proteins are inhibited if actinomycin D is added with the growth effectors. Thus, RNA synthesis appears necessary for the inductions. EGF and the growth inhibitor induce these secreted proteins by independent and noninteracting pathways.