Effect of high pressure on the absorption spectrum and isomeric composition of bacteriorhodopsin.
Effect of high pressure on the absorption spectrum and isomeric composition of bacteriorhodopsin.
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DOI：
10.1016/s0006-3495(80)85083-1
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发表时间：
1980-04
影响因子：
3.4
通讯作者：
M. Tsuda;T. Ebrey
中科院分区：
文献类型：
--
作者：
M. Tsuda;T. Ebrey
研究了高压对暗光型（bRD）和适应光型（bRL）细菌视紫红质的吸收光谱和异构体组成的影响。压力有利于13-顺式细菌视紫红质（br13 -顺式）。在给定压力下，从适应光或暗的细菌视紫质开始，细菌视紫质样品的平衡异构体组成和吸收光谱相同。从压力对br13 -顺式br13 -trans平衡常数的影响可知，bR13-cis与bRall-trans的摩尔体积变化为-7.8 +/- 3.2 ml/mol。这种体积变化表明暗适应和光适应细菌视紫红质的构象存在差异，但变化幅度很小，仅涉及少量蛋白质残基。
The effects of high pressure upon the absorption spectra and isomeric composition of the dark (bRD) and light adapted (bRL) forms of bacteriorhodopsin were examined. Pressure favors the 13-cis form of bacteriorhodopsin (bR13-cis). The equilibrium isomeric composition and absorption spectra of bacteriorhodopsin samples at a given pressure were the same starting from either light or dark adapted bacteriorhodopsin. From the effect of pressure on the equilibrium constant between bRall-trans in equilibrium bR13-cis in the dark, the molar volume change between bRall-trans and bR13-cis was found to be -7.8 +/- 3.2 ml/mol. This volume change suggests a difference in conformation between dark- and light-adapted bacteriorhodopsin, but the magnitude of the change is small, involving only a small number of the protein residues.