HLA- B*5701 typing:: evaluation of an allele-specific polymerase chain reaction melting assay
HLA- B*5701 typing:: evaluation of an allele-specific polymerase chain reaction melting assay
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DOI：
10.1111/j.1399-0039.2007.00840.x
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发表时间：
2007-07-01
期刊：
影响因子：
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通讯作者：
Mallal, S.
中科院分区：
文献类型：
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作者：
Hammond, E.;Mamotte, C.;Mallal, S.
HLA-B*5701的遗传是对抗HIV药物阿巴卡韦过敏反应的强预测因子。因此，在阿巴卡韦治疗机构之前识别易感个体具有临床重要性，并且产生了对携带HLA-B*5701的简单快速诊断测试的需求。在这项研究中，我们描述了这样一种方法的基础上等位基因特异性聚合酶链反应（AS-PCR）和熔解曲线分析的发展。分析了96个患者样本，包括36个HLA-B*5701阳性样本和60个HLA-B*5701阴性样本。与序列分型法相比，该方法对HLA-B*5701等位基因的敏感性和特异性均为100%。总之，AS-PCR/熔解曲线方法最大限度地减少了聚合酶链式反应后的处理过程，并为目前描述的AS-PCR方法提供了一种有吸引力的替代方法。
Inheritance of HLA-B*5701 is a strong predictor of a hypersensitivity reaction to the anti-HIV drug abacavir. The identification of susceptible individuals prior to the institution of abacavir therapy is therefore of clinical importance and has generated demand for a simple and rapid diagnostic test for carriage of HLA-B*5701. In this study, we describe the development of such a method based on allele-specific polymerase chain reaction (AS-PCR) and melting curve analysis. Ninety-six patient samples including 36 HLA-B*5701-positive samples and 60 HLA-B*5701-negative samples were analysed. Compared with sequence-based typing, this method had 100% sensitivity and specificity for the HLA-B*5701 allele. In conclusion, the AS-PCR/melting curve approach minimises post-polymerase chain reaction handling processing and provides an attractive alternative to currently described AS-PCR methods.