A-Single Spermatogonia Heterogeneity and Cell Cycles Synchronize with Rat Seminiferous Epithelium Stages VIII-IX
A-Single Spermatogonia Heterogeneity and Cell Cycles Synchronize with Rat Seminiferous Epithelium Stages VIII-IX
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DOI：
10.1095/biolreprod.113.113555
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发表时间：
2014-02-01
影响因子：
3.6
通讯作者：
Hamra, F. Kent
中科院分区：
文献类型：
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作者：
Abid, Shadaan N.;Richardson, Timothy E.;Hamra, F. Kent
在哺乳动物睾丸中，“A单”精原细胞作为干细胞发挥功能，维持精子产生，以产生卵子。然而，目前还不清楚细胞小生境如何调节A-单精原细胞的发育命运。在此，大鼠睾丸的免疫标记研究定义了一个新的ERBB 3（+）生殖细胞群体，约占总SNAP 91（+）A-单个精原细胞沿着生精波的5%。作为时间的函数，ERBB 3（+）A-单个精原细胞在每个12.9天的精子周期的1- 2天期间被检测到，代表生精上皮阶段VIII-IX中SNAP 91（+）A-单个精原细胞的35%-40%。ERBB 3（+）A-单个精原细胞的局部浓度维持在相邻SNAP 91（+）A-单个精原细胞测量的平均密度以下，可能表明生态位饱和。ERBB 3（+）精原细胞也使其细胞周期与上皮细胞阶段VIII-IX同步，在那里它们与穿过血-睾丸屏障的前细线期精母细胞和进行精子释放的支持细胞形成物理联系。因此，A-单一精原细胞异质性在这个短暂的和重复发生的微环境调用新的理论，细胞壁龛如何整合与睾丸生理学，以协调精子发育的哺乳动物。
In mammalian testes, "A-single'' spermatogonia function as stem cells that sustain sperm production for fertilizing eggs. Yet, it is not understood how cellular niches regulate the developmental fate of A-single spermatogonia. Here, immunolabeling studies in rat testes define a novel population of ERBB3(+) germ cells as approximately 5% of total SNAP91(+) A-single spermatogonia along a spermatogenic wave. As a function of time, ERBB3(+) A-single spermatogonia are detected during a 1- to 2-day period each 12.9-day sperm cycle, representing 35%-40% of SNAP91(+) A-single spermatogonia in stages VIII-IX of the seminiferous epithelium. Local concentrations of ERBB3(+) A-single spermatogonia are maintained under the mean density measured for neighboring SNAP91(+) A-single spermatogonia, potentially indicative of niche saturation. ERBB3(+) spermatogonia also synchronize their cell cycles with epithelium stages VIII-IX, where they form physical associations with preleptotene spermatocytes transiting the blood-testis barrier and Sertoli cells undergoing sperm release. Thus, A-single spermatogonia heterogeneity within this short-lived and reoccurring microenvironment invokes novel theories on how cellular niches integrate with testicular physiology to orchestrate sperm development in mammals.