Fluorometry of hydrogen peroxide using oxidative decomposition of folic acid
Fluorometry of hydrogen peroxide using oxidative decomposition of folic acid
复制标题
DOI：
10.1007/s00216-006-0649-1
复制
发表时间：
2006-08
影响因子：
4.3
通讯作者：
Kazutaka Hirakawa
中科院分区：
文献类型：
--
作者：
Kazutaka Hirakawa
过氧化氢（H2 O2）是最重要的活性氧物种之一。本研究建立了一种叶酸荧光法检测H2 O2的方法。叶酸在Cu（II）存在下被H2 O2分解为蝶呤-6-羧酸，荧光增强。在没有金属离子存在的情况下，超氧阴离子和H2 O2不能分解叶酸。此外，H2 O2加次氯酸钠（单线态氧的来源）不能诱导荧光增强。这些结果表明，H2 O2可以选择性地检测使用叶酸加Cu（II）。H2 O2的检测限（LOD; S/N=3）为0.5 μM。该方法基于叶酸的荧光增强作用，用于测定致癌化合物氨基脲自氧化产生的少量H2 O2（生成速率：≤ 0.01 μM min−1）。
Hydrogen peroxide (H2O2) is one of the most important reactive oxygen species. In the present study, a fluorometry method for detecting H2O2utilizing folic acid was evaluated. Folic acid was decomposed by H2O2in the presence of Cu(II) into pterine-6-carboxylic acid, leading to strong fluorescence enhancement. In the absence of the metal ion, superoxide and H2O2could not decompose folic acid. Also, H2O2plus sodium hypochlorite (a source of singlet oxygen) could not induce fluorescence enhancement. These results demonstrate that H2O2can be selectively detected using folic acid plus Cu(II). The limit of detection (LOD; at S/N=3) for H2O2is 0.5 μM. This method based on the fluorescence enhancement of folic acid was applied in order to determine small amounts of H2O2generated through the autooxidation of semicarbazide (generation rate: ∼0.01 μM min−1), a carcinogenic compound.