Adrenal cortical cell transplantation
Adrenal cortical cell transplantation
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DOI：
10.1016/j.jpedsurg.2004.08.006
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发表时间：
2004-12-01
影响因子：
2.4
通讯作者：
McCabe, ER
中科院分区：
文献类型：
--
作者：
Dunn, JCY;Chu, YT;McCabe, ER
目的：肾上腺皮质是下丘脑-垂体-肾上腺/性腺轴的重要组成部分，协调应激反应并维持体内平衡。作者推测，肾上腺皮质细胞可以移植到肾上腺功能不全状态下再生肾上腺皮质。将在胶原基质中培养的细胞移植到肾包膜下。植入物在1、2、4和8周后取出。从取出的标本中提取总RNA，并通过实时聚合酶链反应进行分析。在取出植入物时，在肾包膜下可观察到明显的类器官。移植后8周，移植物中可检测到Sf 1、Dax 1、星星、Cyp 11 a、Cyp 11 b 1和Cyp 21等肾上腺特异性标志物的表达。肾上腺皮质细胞移植后的体外基因转移可能导致肾上腺功能不全患者的治愈性治疗。(C)2004年爱思唯尔公司All rights reserved.
Purpose: The adrenal cortex is a critical component of the hypothalamic-pituitary-adrenal/gonadal axis that coordinates the stress response and maintains homeostasis. The authors hypothesize that adrenal cortical cells can be transplanted in adrenal insufficiency states to regenerate the adrenal cortex.Methods: Murine adrenal glands were dissociated into adrenal cortical cells. Cells cultured in a collagen matrix were transplanted under the renal capsule. The implants were retrieved 1, 2, 4, and 8 weeks later. Total RNA was extracted from the retrieved specimens and was analyzed by real-time polymerase chain reaction.Results: All animals survived the surgical procedure. At implant retrieval, a distinct organoid could be visualized under the renal capsule. The expressions of adrenal-specific markers including Sf1, Dax1, Star, Cyp11a, Cyp11b1, and Cyp21 were detectable in the retrieved specimens up to 8 weeks posttransplantation.Conclusion: Primary adrenal cortical cells retained their gene expressions after heterotopic transplantation. Ex vivo gene transfer followed by adrenal cortical cell transplantation may lead to curative therapy for patients with adrenal insufficiency. (C) 2004 Elsevier Inc. All rights reserved.