Electrospun porous La–Sr–Co–Ni–O nanofibers for highly sensitive non-enzymatic glucose detection
Electrospun porous La–Sr–Co–Ni–O nanofibers for highly sensitive non-enzymatic glucose detection
复制标题
电纺多孔La-Sr-Co-Ni-O纳米纤维用于高灵敏非酶葡萄糖检测
DOI：
10.1039/d1ma00984b
复制
发表时间：
2022
影响因子：
5
通讯作者：
Han, Shubo
中科院分区：
文献类型：
--
作者：
Pelucarte, Kasci D.;Hatchell, Tashi A.;George, Gibin;Ede, Sivasankara Rao;Adhikari, Menuka;Lin, Yulin;Wen, Jianguo;Luo, Zhiping;Han, Shubo
葡萄糖生物传感器广泛用于临床、工业和环境应用。基于具有钙钛矿结构的金属氧化物的非酶电化学葡萄糖生物传感器具有高灵敏度、优异的稳定性和成本效益。通过静电纺丝优化A位和B位元素，并在700 °C下煅烧5 h，制备了具有ABO 3型钙钛矿结构的多孔La-Sr-Co-Ni-O（LSCNO）纳米纤维。通过扫描和透射电子显微镜、X射线衍射和X射线光电子能谱表征，证实所制备的纳米纤维是多孔的、纳米尺寸的多晶颗粒，具有高结晶度。研制了一种新型的基于La0.75Sr0.25Co0.5Ni0.5O3的非酶电化学传感器，该传感器对葡萄糖具有敏感性，其电化学催化机理为Ni（II）/Ni（III）或Co（II）/Co（III）介导的电子转移，并伴随葡萄糖酸络合作用.葡萄糖生物传感器在0.1-1.0 mM范围内呈现线性响应，校准灵敏度为924 ± 28 μA mM−1，方差比例为0.9926，检测下限为0.083 mM，分别显示出出色的分析性能。该生物传感器对最广泛使用的阴离子表面活性剂十二烷基硫酸钠没有反应，对其他生物分子如果糖、乳糖、半乳糖、甘露糖、多巴胺和抗坏血酸的灵敏度较低。用该传感器对尿样进行标准加入法检测，表明该传感器具有潜在的临床应用价值。
Glucose biosensors are widely used for clinical, industrial, and environmental applications. Nonenzymatic electrochemical glucose biosensors based on metal oxides with a perovskite structure have exhibited high sensitivity, excellent stability, and cost efficiency. In this work, porous La–Sr–Co–Ni–O (LSCNO) nanofibers, with an ABO3-type perovskite structure, were prepared through optimizing the A-site and B-site elements by electrospinning, followed with calcination at 700 °C for 5 h. Characterized by scanning and transmission electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy, fabricated nanofibers were confirmed to be porous and nanosized polycrystalline grains with high crystallinity. A novel La0.75Sr0.25Co0.5Ni0.5O3-based nonenzymatic electrochemical biosensor was developed, which is sensitive to glucose because of an electrochemically catalytic mechanism, a mediated electron transfer involving Ni(II)/Ni(III) or Co(II)/Co(III), accompanying with gluconic acid complexation. The glucose biosensor presented a linear response in the range of 0.1–1.0 mM with a calibration sensitivity of 924 ± 28 μA mM−1, a proportion of the variance of 0.9926, and a lower limit of detection of 0.083 mM, respectively, demonstrating an outstanding analytical performance. The biosensor showed no response to the most widely used anionic surfactant, sodium dodecyl sulfate, and low sensitivity to other biomolecules, such as fructose, lactose, galactose, mannose, dopamine, and ascorbic acid. A urine sample was tested by this novel nonenzymatic electrochemical biosensor by standard addition method, suggesting a potential application for clinical test.