A 5-month period of epilepsy impairs spatial memory, decreases anxiety, but spares object recognition in the lithium-pilocarpine model in adult rats
A 5-month period of epilepsy impairs spatial memory, decreases anxiety, but spares object recognition in the lithium-pilocarpine model in adult rats
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DOI：
10.1111/j.0013-9580.2005.38704.x
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发表时间：
2005-04-01
期刊：
影响因子：
5.6
通讯作者：
Nehlig, A
中科院分区：
文献类型：
--
作者：
Detour, J;Schroeder, H;Nehlig, A
目的：在颞叶癫痫 (TLE) 中，发作间期行为障碍会影响患者的生活质量。因此，我们研究了 TLE 的锂-毛果芸香碱 (li-pilo) 模型中的长期行为障碍。方法：将 11 只在 5 个月内表现出自发性复发性癫痫发作 (SRS) 的 li-pilo 成年大鼠与 11 只 li-saline 大鼠进行比较。在径向臂迷宫（RAM）中测试空间工作记忆，在高架十字迷宫（EPM）中测试焦虑，在物体识别范例中测试非空间工作记忆。行为测试后，对硫堇脑切片的神经元损失进行评估。结果：在 RAM 中，li-saline 大鼠的完成每次训练的时间和每次训练的错误数量在 5 天的时间内有所减少，但在 li-pilo 大鼠中保持恒定且显着较高。在 EPM 中，li-pilo 大鼠的进入次数和张开手臂所花费的时间显着高于 li-saline 大鼠。在物体识别任务中，两组人对新物体表现出相当的新奇偏好。门、CA1、杏仁核、梨状皮层和内嗅皮层的神经元损失达到 47-90%。结论：在经历了 5 个月的 SRS 的 li-pilo 大鼠中，物体识别任务的表现没有受到影响，这表明尽管受到广泛损伤，物体辨别能力仍然相对完整。介导记忆和焦虑的区域（例如海马体、内嗅皮层和杏仁核）的神经元损失可能与空间定向受损和焦虑减少有关。
Purpose: In temporal lobe epilepsy (TLE), interictal behavioral disorders affect patients' quality of life. Therefore we studied long-term behavioral impairments in the lithium-pilocarpine (li-pilo) model of TLE.Methods: Eleven li-pilo adult rats exhibiting spontaneous recurrent seizures (SRSs) during 5 months were compared with 11 li-saline rats. Spatial working memory was tested in a radial arm maze (RAM), anxiety in an elevated plus-maze (EPM), and nonspatial working memory in an object-recognition paradigm. Neuronal loss was assessed on thionine brain sections after behavioral testing.Results: In the RAM, the time to complete each session and the number of errors per session decreased over a 5-day period in li-saline rats but remained constant and significantly higher in li-pilo rats. In the EPM, the number of entries in and time spent on open arms were significantly higher in li-pilo than li-saline rats. In the object-recognition task, the two groups exhibited a comparable novelty preference for the new object. Neuronal loss reached 47-90% in hilus, CA1, amygdala, and piriform and entorhinal cortex.Conclusions: In li-pilo rats having experienced SRS for 5 months, performance in the object-recognition task is spared, which suggests that object discrimination remains relatively intact despite extensive damage. Neuronal loss in regions mediating memory and anxiety, such as hippocampus, entorhinal cortex, and amygdala, may relate to impaired spatial orientation and decreased anxiety.