Organization and evolution of four differentially amplified tandem repeats in the Cucumis hystrix genome
Organization and evolution of four differentially amplified tandem repeats in the Cucumis hystrix genome
复制标题
黄瓜基因组中四个差异扩增串联重复的组织和进化
DOI：
10.1007/s00425-017-2716-6
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发表时间：
2017
期刊：
影响因子：
4.3
通讯作者：
Chen JF
中科院分区：
文献类型：
--
作者：
Yang Shuqiong;Qin Xiaodong;Cheng Chunyan;Li Ziang;Lou Qunfeng;Li Ji;Chen Jinfeng;Chen JF
主要结论子宫颈3个亚端粒卫星和1个间质5S rDNA，以及2个oc的近中心粒信号。亚端粒卫星。sativuschromosome支持了inCucumis染色体融合假说。串联重复序列是在大多数真核生物中以大串联阵列组织的高度重复序列组成的染色体结构部分。串联重复序列的分化直接影响染色体结构，从而影响物种的形成和进化。黄瓜hystrix（2n= 2x= 24）是唯一与c属同属的野生黄瓜种。sativus(2n= 2x= 14)，因此其系统发育位置赋予c至关重要的作用。了解人的染色体进化。然而，我们对c。尾串联重复序列不足以详细了解兔的染色体进化。基于生物信息学和原位杂交（ISH）技术，我们鉴定并鉴定了4个差异扩增的串联重复序列：黄瓜hystrixsatellite 1-3 （CuhySat1-CuhySat3）位于所有染色体的亚端粒区，黄瓜hystrix5S （Cuhy5S）位于单个染色体对的间隙区。利用CuhySat1-3和Cuhy5S比对ISH图谱，发现c - 3和Cuhy5S之间串联重复序列具有高度同源性。hystrixandC。巨大成功。有趣的是，我们发现了CuhySat2和CuhySat3在c上的信号分布变化。sativuschromosomes。比较它们在c上的亚端粒信号分布。结果表明，CuhySat3在hstrixxchromosome上表现为端粒内信号分布，CuhySat2在c上表现为亚端粒和端粒内信号分布。sativuschromosomes。这是详细的表征。hystrixtandem repeat显著拓宽了我们对c的认识。观察到的信号分布变化将有助于了解黄瓜染色体的进化。
Main conclusionThree subtelomeric satellites and one interstitial 5S rDNA were characterized inCucumis hystrix, and the pericentromeric signals of twoC. hystrixsubtelomeric satellites alongC. sativuschromosomes supported the hypothesis of chromosome fusion inCucumis.Tandem repeats are chromosome structural fractions consisting of highly repetitive sequences organized in large tandem arrays in most eukaryotes. Differentiation of tandem repeats directly affects the chromosome structure, which contributes to species formation and evolution.Cucumis hystrix(2n= 2x= 24) is the only wildCucumisspecies grouped into the same subgenus withC. sativus(2n= 2x= 14), hence its phylogenetic position confers a vital role forC. hystrixto understand the chromosome evolution inCucumis. However, our knowledge ofC. hystrixtandem repeats is insufficient for a detailed understanding of the chromosome evolution inCucumis. Based on de novo tandem repeat characterization using bioinformatics and in situ hybridization (ISH), we identified and characterized four differentially amplified tandem repeats,Cucumis hystrixsatellite 1–3 (CuhySat1–CuhySat3) located at the subtelomeric regions of all chromosomes, andCucumis hystrix5S (Cuhy5S) located at the interstitial regions of one single chromosome pair. Comparative ISH mapping using CuhySat1–3 and Cuhy5S revealed high homology of tandem repeats betweenC. hystrixandC. sativus. Intriguingly, we found signal distribution variations of CuhySat2 and CuhySat3 onC. sativuschromosomes. In comparison to their subtelomeric signal distribution onC. hystrixchromosomes, CuhySat3 showed a pericentromeric signal distribution and CuhySat2 showed both subtelomeric and pericentromeric signal distributions onC. sativuschromosomes. This detailed characterization of fourC. hystrixtandem repeats significantly widens our knowledge of theC. hystrixchromosome structure, and the observed signal distribution variations will be helpful for understanding the chromosome evolution ofCucumis.