Tol2 transposon-mediated transgenesis in Xenopus tropicalis
Tol2 transposon-mediated transgenesis in Xenopus tropicalis
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DOI：
10.1002/dvg.20234
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发表时间：
2006-09-01
期刊：
影响因子：
1.5
通讯作者：
Mead, Paul E.
中科院分区：
文献类型：
--
作者：
Hamlet, Michelle R. Johnson;Yergeau, Donald A.;Mead, Paul E.
二倍体热带爪蟾（Xenopus tropicalis）正在成为一个强大的发育遗传模型系统。热带假单胞菌基因组测序已接近完成，几个实验室正在进行诱变筛选。我们有兴趣在热带爪蟾中开发插入突变策略。转座子介导的插入突变，曾经只用于植物和无脊椎动物系统，现在更广泛地适用于脊椎动物。开发转座子作为诱变工具的第一步是证明这些移动的元件在靶生物中有效地发挥作用。在这里，我们表明，青鳉鱼转座子，Tol2，能够稳定地整合到X。tropicalis基因组，并将作为一个强大的工具，插入诱变策略的青蛙。
The diploid frog Xenopus tropicalis is becoming a powerful developmental genetic model system. Sequencing of the X tropicalis genome is nearing completion and several labs are embarking on mutagenesis screens. We are interested in developing insertional mutagenesis strategies in X tropicalis. Transposon-mediated insertional mutagenesis, once used exclusively in plants and invertebrate systems, is now more widely applicable to vertebrates. The first step in developing transposons as tools for mutagenesis is to demonstrate that these mobile elements function efficiently in the target organism. Here, we show that the Medaka fish transposon, Tol2, is able to stably integrate into the X. tropicalis genome and will serve as a powerful tool for insertional mutagenesis strategies in the frog.