Evaluation of cyclin D1 expression and its subcellular distribution in mouse tissues
Evaluation of cyclin D1 expression and its subcellular distribution in mouse tissues
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DOI：
10.1111/j.0021-8782.2004.00347.x
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发表时间：
2004-11-01
影响因子：
2.4
通讯作者：
De Luca, A
中科院分区：
文献类型：
--
作者：
De Falco, M;Fedele, V;De Luca, A
细胞周期蛋白D1是细胞通过G1期进入S期所需的关键细胞周期调节蛋白。我们通过Western印迹分析表明，细胞周期蛋白D1在成年小鼠组织中广泛分布，其表达水平具有组织依赖性。免疫组织化学研究还表明，细胞周期蛋白D1可能存在于胞浆、胞核或两个胞室中：在增殖细胞(如肾、肠、胃和唾液腺)和未分裂细胞(成人脑的成熟神经元)中均可见胞浆染色，而在胚胎神经系统的神经元中可见核染色。免疫电子显微镜结果表明，在细胞周期蛋白D1存在于两个隔室的组织中(例如肠上皮细胞)，它可能通过核孔从细胞核移动到细胞质，反之亦然。作为一个整体，这些发现表明细胞周期蛋白D1可能通过与不同底物相互作用在特定组织中扮演多种角色，其在核间和细胞质间的转移可能有助于维持细胞内环境的稳定。
Cyclin D1 is a key cell-cycle regulatory protein required for the cell to progress through G1 to S phase. We have shown by Western blot analysis that cyclin D1 has a wide distribution in adult mouse tissues, with its level of expression being tissue-dependent. Immunohistochemistry has also shown that cyclin D1 may be present in the cytoplasm, in the nucleus or in both these cell compartments: cytoplasmic staining was observed in both proliferating cells (e.g. kidney, intestine, stomach and salivary gland) and in the non-dividing cells (the mature neurons of adult brain), while nuclear staining was seen in the neurons of the embryonic nervous system. Immunoelectron microscopy results indicate that, in tissues where cyclin D1 is present in both compartments (e.g. intestinal enterocytes), it may move via nuclear pores from the nucleus to the cytoplasm, and vice versa. The findings as a whole suggest that cyclin D1 may play multiple roles within specific tissues, probably by interacting with different substrates, and that its transit between nuclear and cytoplasmic compartments may help maintain cell homeostasis.