ISOLATION AND IDENTIFICATION OF 4-HYDROXYRETINOIC AND 4-OXORETINOIC ACID - INVITRO METABOLITES OF ALL-TRANS-RETINOIC ACID IN HAMSTER TRACHEA AND LIVER
ISOLATION AND IDENTIFICATION OF 4-HYDROXYRETINOIC AND 4-OXORETINOIC ACID - INVITRO METABOLITES OF ALL-TRANS-RETINOIC ACID IN HAMSTER TRACHEA AND LIVER
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DOI：
10.1021/bi00577a039
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发表时间：
1979-01-01
期刊：
影响因子：
2.9
通讯作者：
SPORN, MB
中科院分区：
文献类型：
--
作者：
FROLIK, CA;ROBERTS, AB;SPORN, MB
Incubation of [3H]retinoic acid in the presence of hamster liver 10,000 g supernatant produces several metabolites that are more polar than the parent compound. Two of these metabolites are identical with synthetic all-trans-4-hydroxyretinoic acid and all-trans-4-oxoretinoic acid both in UV absorption and mass spectral characteristics and in migration rates on 2 different reverse-phase, high-pressure liquid chromatographic (HPLC) systems. The metabolites produced in a cell-free liver incubation reaction also migrate on a HPLC column together with metabolites isolated from a tracheal organ culture system. Both the metabolites and the synthetic standards show less biological activity than the parent all-trans-retinoic acid in a tracheal organ culture assay.
