Dense transient receptor potential cation channel, vanilloid family, type 2 (TRPV2) immunoreactivity defines a subset of motoneurons in the dorsal lateral nucleus of the spinal cord, the nucleus ambiguus and the trigeminal motor nucleus in rat
Dense transient receptor potential cation channel, vanilloid family, type 2 (TRPV2) immunoreactivity defines a subset of motoneurons in the dorsal lateral nucleus of the spinal cord, the nucleus ambiguus and the trigeminal motor nucleus in rat
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DOI：
10.1016/j.neuroscience.2007.09.073
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发表时间：
2008-01-02
期刊：
影响因子：
3.3
通讯作者：
Basbauma, A. I.
中科院分区：
文献类型：
--
作者：
Lewinter, R. D.;Scherrer, G.;Basbauma, A. I.
瞬时受体电位阳离子通道，vanilloid family, type 2 （TRPV2）是TRPV蛋白家族的成员，是辣椒素/香草素受体（瞬时受体电位阳离子通道，vanilloid family, type 1, TRPV1）的同源物。与TRPV1一样，TRPV2在投射到脊髓背角浅层的背根神经节（DRG）神经元亚群中表达。由于毒热（> - 52℃）激活了转染细胞中的TRPV2，该通道与体内高强度热痛信息的处理有关。然而，与TRPV1仅限于小直径DRG神经元相反，TRPV2在中枢神经系统的各个区域都有显著的免疫反应性。本报告主要关注大鼠脑干和脊髓中的神经元子集，包括脊髓背外侧核（DLN）、歧义核和运动三叉核。双标记免疫细胞化学与运动神经元的标记，结合逆行标记，确定这些细胞，实际上是运动神经元。除了直径较小外，这些细胞与其他运动神经元没有什么不同，后者只有轻微的trpv2免疫反应。对于大多数DLN神经元，密集标记的群体共表达雄激素受体，并遵循正常的DLN个体发育。TRPV2在这三种不同的脊髓和脑干运动神经元组中非常强烈的表达的功能意义仍有待确定。(c) 2008 ibro。Elsevier Ltd.出版。版权所有。
The transient receptor potential cation channel, vanilloid family, type 2 (TRPV2) is a member of the TRPV family of proteins and is a homologue of the capsaicin/vanilloid receptor (transient receptor potential cation channel, vanilloid family, type 1, TRPV1). Like TRPV1, TRPV2 is expressed in a subset of dorsal root ganglia (DRG) neurons that project to superficial laminae of the spinal cord dorsal horn. Because noxious heat (>52 degrees C) activates TRPV2 in transfected cells this channel has been implicated in the processing of high intensity thermal pain messages in vivo. In contrast to TRPV1, however, which is restricted to small diameter DRG neurons, there is significant TRPV2 immunoreactivity in a variety of CNS regions. The present report focuses on a subset of neurons in the brainstem and spinal cord of the rat including the dorsal lateral nucleus (DLN) of the spinal cord, the nucleus ambiguus, and the motor trigeminal nucleus. Double label immunocytochemistry with markers of motoneurons, combined with retrograde labeling, established that these cells are, in fact, motoneurons. With the exception of their smaller diameter, these cells did not differ from other motoneurons, which are only lightly TRPV2-immunoreactive. As for the majority of DLN neurons, the densely-labeled populations co-express androgen receptor and follow normal DLN ontogeny. The functional significance of the very intense TRPV2 expression in these three distinct spinal cord and brainstem motoneurons groups remains to be determined. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.