Wolbachia density and virulence attenuation after transfer into a novel host
Wolbachia density and virulence attenuation after transfer into a novel host
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DOI：
10.1073/pnas.052466499
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发表时间：
2002-03-05
影响因子：
11.1
通讯作者：
O'Neill, SL
中科院分区：
文献类型：
--
作者：
McGraw, EA;Merritt, DJ;O'Neill, SL
考虑到共生体和宿主基因型的复杂相互作用，控制胞内细菌Wolbachia pipientis在其昆虫宿主中复制速率的因素是未知的，难以探索。使用一个菌株的沃尔巴克氏体，是已知的过度复制和缩短寿命的果蝇宿主，我们已经跟踪了体细胞和生殖组织中的一个新的主机/沃尔巴克氏体协会复制控制的演变。将来自D.通过将后腹果蝇（metanogaster）与拟果蝇（Drosophila simulans）进行比较，我们证明了卵巢中最初的高密度超过了完美的母体传播所需的密度，并且可能导致生殖适合度的降低。与卵巢感染相关的密度和健身成本迅速下降，在几代后transinfection。D. simulans仅轻微衰减，与D.后胃这项研究揭示了一个强大的主机参与沃尔巴克氏体的复制率，在不同组织中的密度控制反应的独立性，以及自然选择的力量作用于生殖健康。
The factors that control replication rate of the intracellular bacterium Wolbachia pipientis in its insect hosts are unknown and difficult to explore, given the complex interaction of symbiont and host genotypes. Using a strain of Wolbachia that is known to over-replicate and shorten the lifespan of its Drosophila melanogaster host, we have tracked the evolution of replication control in both somatic and reproductive tissues in a novel host/Wolbachia association. After transinfection (the transfer of a Wolbachia strain into a different species) of the over-replicating Wolbachia popcorn strain from D. metanogaster to Drosophila simulans, we demonstrated that initial high densities in the ovaries were in excess of what was required for perfect maternal transmission, and were likely causing reductions in reproductive fitness. Both densities and fitness costs associated with ovary infection rapidly declined in the generations after transinfection. The early death effect in D. simulans attenuated only slightly and was comparable to that induced in D. metanogaster. This study reveals a strong host involvement in Wolbachia replication rates, the independence of density control responses in different tissues, and the strength of natural selection acting on reproductive fitness.