Flagellar waveforms of gametes in the brown alga Ectocarpus siliculosus.
Flagellar waveforms of gametes in the brown alga Ectocarpus siliculosus.
复制标题
褐藻 Ectocarpus siliculusus 配子的鞭毛波形。
DOI：
10.1080/09670262.2015.1109144
复制
发表时间：
2016
影响因子：
2.4
通讯作者：
Nagasato C and Motomura T.
中科院分区：
文献类型：
--
作者：
Kinoshita N;Shiba K;Inaba K;Fu G;Nagasato C and Motomura T.
褐藻是层藻纲的成员，它们的配子通常具有两个异质鞭毛：长的前鞭毛（AF）和短的后鞭毛（PF）。在这项研究中，游泳路径和鞭毛的波形在自由游泳和触泳的男性和女性配子，并在男性配子在趋化过程中，定量分析了模型棕色algaEctocarpus siliculosus。该分析使用高速摄像机进行。结果表明，AF在改变雄配子和雌配子的运动从自由游泳到触泳，以及在改变雄配子的游泳路径从线性到圆形的趋化过程中发挥作用。在性信息素存在下，雄配子的游动路径由直线（游动路径曲率，0-0.02 µm-1）变为中、小圆形（游动路径曲率，0.04-0.20 µm-1）。鞭毛的不对称性和偏转角的AF变得更大，而AF的振荡模式是稳定的。而当雄配子呈中、小圆形轨迹时，鞭毛的不对称性与鞭毛偏转角和轨迹曲率无关。PF不规则地改变偏转角和振荡模式是不稳定的依赖于性信息素浓度的梯度。AF波形是独立的PF运动在趋化。这意味着AF有能力改变雄配子的游动路径-例如，从高度线性路径到圆形路径-而从中间圆形路径到小圆形路径的运动变化是PF跳动的结果。
Brown algae are members of the Stramenopiles and their gametes generally have two heterogeneous flagella: a long anterior flagellum (AF) with mastigonemes and a short posterior flagellum (PF). In this study, swimming paths and flagellar waveforms in free-swimming and thigmotactic-swimming male and female gametes and in male gametes during chemotaxis, were quantitatively analysed in the model brown algaEctocarpus siliculosus. This analysis was performed using a high-speed video camera. It was revealed that the AF plays a role in changing the locomotion of male and female gametes from free-swimming to thigmotactic-swimming and also in changing the swimming path of male gametes from linear to circular during chemotaxis. In the presence of a sex pheromone, male gametes changed their swimming path from linear (swimming path curvature, 0–0.02 µm–1) to middle and small circular path (swimming path curvature, 0.04–0.20 µm–1). The flagellar asymmetry and the deflection angle of the AF became larger, whereas the oscillation pattern of the AF was stable. However, there was no correlation between the flagellar asymmetry and the deflection angle of the AF and the path curvature when the male gametes showed middle to small circular paths. The PF irregularly changed the deflection angle and the oscillation pattern was unstable depending on the gradient of the sex pheromone concentration. AF waveforms were independent of PF locomotion during chemotaxis. This means that the AF has the ability to change the swimming path of male gametes – for example, from a highly linear path to a circular path – while changes in locomotion from a middle circle path to a small circle path is the result of beating of the PF.