Mapping In Vivo Protein-DNA Interactions in Plants by DamID, a DNA Adenine Methylation-Based Method
Mapping In Vivo Protein-DNA Interactions in Plants by DamID, a DNA Adenine Methylation-Based Method
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DOI：
10.1007/978-1-61779-154-3_18
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Gaudin, Valerie
中科院分区：
文献类型：
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作者：
Germann, Sophie;Gaudin, Valerie
DamID (DNA adenine methylation identification) is an adenine methylation-based tagging method designed to map protein-DNA interactions in vivo. Dam ID, an alternative method to chromatin immunoprecipitation (ChIP), is based on the covalent linking of a "fingerprint" in the vicinity of the DNA-binding sites of the protein of interest. The fingerprints can be further mapped by simple molecular approaches. First developed by van Steensel's group in Drosophila melano,gaster (1), DamID was successfully adapted to Arabidopsis thaliana, and its feasibility demonstrated by using the well-known yeast GAL4 transcription factor (2). The method was further used to establish a genome-wide map of the target sites of LHPI, a regulatory chromatin protein in A. thaliana (3).
