PHASE VARIATION OF TYPE-1 FIMBRIAE IN ESCHERICHIA-COLI IS UNDER TRANSCRIPTIONAL CONTROL
PHASE VARIATION OF TYPE-1 FIMBRIAE IN ESCHERICHIA-COLI IS UNDER TRANSCRIPTIONAL CONTROL
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DOI：
10.1126/science.6116279
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发表时间：
1981-01-01
期刊：
影响因子：
56.9
通讯作者：
EISENSTEIN, BI
中科院分区：
文献类型：
--
作者：
EISENSTEIN, BI
在大肠杆菌K12株中实现了lac基因与1型菌毛（皮利）合成所需基因的操纵子融合。由噬菌体mu d（Apr（氨苄青霉素抗性），lac）溶原化的大肠杆菌。合成β-半乳糖苷酶因此反映了转录因子基因的转录，并被用作菌毛调节的探针。操纵子融合的表达振荡，表明流苏状和非流苏状状态之间的相位变化是在转录控制下。从流苏状到非流苏状的转变率为1.05 × 10 - 5。10 ~（-3）个/菌，从无菌毛到有菌毛，每代3.12 × 10 ~（-3）个。每代10-3个/菌。
An operon fusion of the lac genes to those required for synthesis of type 1 fimbriae (pili) was achieved in a K12 strain of E. coli lysogenized by the bacteriophage mu d (Apr (ampicillin resistant), lac). Synthesis of .beta.-galactosidase thus reflected pil gene transcription and was used as a probe of fimbrial regulation. Expression of the operon fusion oscillated, demonstrating that phase variation between fimbriate and nonfimbriate states is under transcriptional control. The transition rates from fimbriate to nonfimbriate were 1.05 .times. 10-3/bacterium per generation and from nonfimbriate to fimbriate, 3.12 .times. 10-3/bacterium per generation.