Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate
Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate
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DOI：
10.1038/s41598-018-38455-w
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发表时间：
2019-02-12
影响因子：
4.6
通讯作者：
Clarke, Jonathan D. W.
中科院分区：
文献类型：
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作者：
Araya, Claudio;Hakkinen, Hanna-Maria;Clarke, Jonathan D. W.
组织内化是胚胎组织产生复杂内脏器官的关键形态发生机制，许多关于上皮组织的研究概述了组织内化的一般观点。在这里，我们使用了定量的活体成像和突变分析来确定在一个显然没有典型的上皮性组织-斑马鱼神经板的组织中，是否有类似的机制负责内化。我们发现，尽管斑马鱼胚胎在没有常规上皮的情况下开始神经分化，但位于内侧的神经板细胞采用了典型的上皮细胞策略，以便在细胞内化过程中收缩其背部表面膜。此外，我们发现肌球蛋白II的活性是这种瞬时细胞重塑的重要驱动因素，这种重塑也依赖于CDH2(N-钙粘附素)。取消CDH2会导致肌球蛋白-II分布缺陷、错误定位的内化事件和神经板形态发生缺陷。我们的工作表明，CDH2协调斑马鱼神经板上肌球蛋白-II依赖的内化。
Tissue internalisation is a key morphogenetic mechanism by which embryonic tissues generate complex internal organs and a number of studies of epithelia have outlined a general view of tissue internalisation. Here we have used quantitative live imaging and mutant analysis to determine whether similar mechanisms are responsible for internalisation in a tissue that apparently does not have a typical epithelial organisation - the zebrafish neural plate. We found that although zebrafish embryos begin neurulation without a conventional epithelium, medially located neural plate cells adopt strategies typical of epithelia in order to constrict their dorsal surface membrane during cell internalisation. Furthermore, we show that Myosin-II activity is a significant driver of this transient cell remodeling which also depends on Cdh2 (N-cadherin). Abrogation of Cdh2 results in defective Myosin-II distribution, mislocalised internalisation events and defective neural plate morphogenesis. Our work suggests Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate.