PEMBL - A NEW FAMILY OF SINGLE STRANDED PLASMIDS
PEMBL - A NEW FAMILY OF SINGLE STRANDED PLASMIDS
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DOI：
10.1093/nar/11.6.1645
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发表时间：
1983-01-01
影响因子：
14.9
通讯作者：
CORTESE, R
中科院分区：
文献类型：
--
作者：
DENTE, L;CESARENI, G;CORTESE, R
我们构建了一系列质粒，即pEMBL家族，其特点是：1)bla基因作为可选择标记，2)编码β-半乳糖苷酶β-肽的短片段，并含有多个克隆位点的多连接子，3)噬菌体Fl的基因内区。pEMBL质粒在与噬菌体Fl重复感染后，具有被封装为单链DNA的特性。这些载体已成功地用于DNA测序。并可用于使用M13衍生品的任何其他目的。然而，pEMBL质粒具有比Ml3载体更小的优点，并且DNA的纯化更简单。此外，最重要的是，与Ml3载体相比，长插入在pEMBL质粒中具有更高的稳定性。
We have constructed a series of plasmids, the pEMBL family, characterized by the presence of 1) the bla gene as selectable marker, 2) a short segment coding for the β–peptide of β-galactosidase and containing a multiple cloning sites polylinker, 3) the intragenic region of phage Fl.pEMBL plasmids have the property of being encapsidated as single stranded DNA, upon superinfection with phage Fl. These vectors have been used successfully for DNA sequencing with the dideoxy–method, and can be used for any other purpose for which M13 derivatives are used. However, the pEMBL plasmids have the advantage of being smaller than Ml3 vectors, and the purification of the DNA is simpler. In addition, and most importantly, long inserts have a higher stability in pEMBL plasmids than Ml3 vectors.