PLAQUE INHIBITION ASSAY FOR DRUG SUSCEPTIBILITY TESTING OF INFLUENZA-VIRUSES
PLAQUE INHIBITION ASSAY FOR DRUG SUSCEPTIBILITY TESTING OF INFLUENZA-VIRUSES
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DOI：
10.1128/aac.17.5.865
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发表时间：
1980-01-01
影响因子：
4.9
通讯作者：
DOUGLAS, RG
中科院分区：
文献类型：
--
作者：
HAYDEN, FG;COTE, KM;DOUGLAS, RG
通过空斑抑制测定，在 Madin-Darby 犬肾细胞单层中测定了 4 种药物针对当代甲型和乙型流感病毒株的相对抗病毒活性。该测定被证明是一种可靠、快速的确定 50% 抑制浓度的方法，与临床可达到的药物水平和临床试验结果密切相关。现代甲型流感病毒株（H1N1、H3N2、HSW1N1 亚型）需要盐酸金刚烷胺和盐酸金刚乙胺 50% 抑制浓度范围为 0.2-0.4 μg/ml，而 50% 抑制浓度范围为 .apprx。 50-100 .mu.g/ml 针对乙型流感病毒。利巴韦林对甲型流感病毒的活性比盐酸金刚烷胺低约10倍，并且利巴韦林对甲型和乙型流感病毒的50％抑制浓度范围为2.6-6.8μg/ml。 Inosiplex 在此测试系统中没有抗病毒活性。
The relative antiviral activities of 4 drugs against contemporary strains of influenza A and B viruses were determined in Madin-Darby canine kidney cell monolayers with a plaque inhibition assay. This assay proved to be a reliable, rapid method of determining 50% inhibitory concentrations that correlated well with clinically achievable drug levels and the results of clinical trials. Contemporary strains of influenza A viruses (subtypes H1N1, H3N2, HSW1N1) required amantadine hydrochloride and rimantadine hydrochloride 50% inhibitory concentrations in the range of 0.2-0.4 .mu.g/ml, whereas 50% inhibitory concentrations ranged from .apprx. 50-100 .mu.g/ml against influenza B viruses. Ribavirin was approximately 10-fold less active than amantadine hydrochloride against influenza A viruses, and the ribavirin 50% inhibitory concentrations against both influenza A and B viruses ranged from 2.6-6.8 .mu.g/ml. Inosiplex had no antiviral activity in this test system.