Effect of Long-Term and Short-Term Imbalanced Zn Manipulation on Gut Microbiota and Screening for Microbial Markers Sensitive to Zinc Status.
Effect of Long-Term and Short-Term Imbalanced Zn Manipulation on Gut Microbiota and Screening for Microbial Markers Sensitive to Zinc Status.
复制标题
DOI：
10.1128/spectrum.00483-21
复制
发表时间：
2021-12-22
影响因子：
3.7
通讯作者：
Feng J
中科院分区：
文献类型：
--
作者：
Chen L;Wang Z;Wang P;Yu X;Ding H;Wang Z;Feng J
锌 (Zn) 失衡是世界范围内常见的单一营养素紊乱，但人们对膳食锌失衡对肠道微生物组的短期和长期影响知之甚少。在这里，3周大的C57BL/6小鼠被喂食补充有0（低锌）、30（对照锌）、150（高锌）和600毫克/千克体重（过量锌）剂量的锌的饮食，持续4周（短期）和8周（长期）。由于锌饮食不平衡，肠道细菌的门、属和种水平发生了变化（例如罗伊氏乳杆菌和阿克曼氏菌）。此外，短期低锌饮食会减少碳水化合物、聚糖和核苷酸代谢等途径。长期低锌饮食会抑制戊酸的产生。在高锌和过量锌饮食中，耐药性和传染病等途径在 4 周和 8 周的间隔内上调。长期的锌强化剂量，特别是在高锌水平下，抑制了短链脂肪酸（SCFA）产生属的丰度以及代谢物的浓度。最后，Melainabacteria（门）和Desulfovibrio sp。菌株 ABHU2SB（种）被鉴定为高精度 Zn 状态的潜在标记（曲线下面积 [AUC]， >0.8）。总的来说，这项研究发现了改变锌喂养的小鼠肠道微生物组成及其代谢物浓度的显着变化，以及锌状态的相关微生物标志物。重要性 锌缺乏是发展中国家的一个重要健康问题。为了防止锌缺乏的发生，锌强化和补充被广泛使用。然而，在发达国家，锌的摄入量常常超过可耐受的摄入上限。我们的结果表明，膳食锌是微生物群落结构的重要调节剂，锌缺乏和锌过量都会导致肠道微生物群失调。此外，还鉴定了锌状态的特定微生物生物标志物并与血清锌水平相关。我们的研究发现，短期低锌饮食（0mg/kg）和长期高锌饮食（150mg/kg）对小鼠模型有明显的负面影响。因此，这些结果表明补充锌的提供和持续时间应谨慎对待。
Zinc (Zn) imbalance is a common single-nutrient disorder worldwide, but little is known about the short-term and long-term effects of imbalanced dietary zinc in the intestinal microbiome. Here, 3-week-old C57BL/6 mice were fed diets supplemented with Zn at the doses of 0 (low Zn), 30 (control Zn), 150 (high Zn), and 600 mg/kg of body weight (excess Zn) for 4 weeks (short term) and 8 weeks (long term). The gut bacterial composition at the phyla, genus, and species levels were changed as the result of the imbalanced Zn diet (e.g., Lactobacillus reuteri and Akkermansia muciniphila). Moreover, pathways including carbohydrate, glycan, and nucleotide metabolism were decreased by a short-term low-Zn diet. Valeriate production was suppressed by a long-term low-Zn diet. Pathways such as drug resistance and infectious diseases were upregulated in high- and excess-Zn diets over 4-week and 8-week intervals. Long-term zinc fortification doses, especially at the high-Zn level, suppressed the abundance of short-chain fatty acids (SCFAs)-producing genera as well as the concentrations of metabolites. Finally, Melainabacteria (phylum) and Desulfovibrio sp. strain ABHU2SB (species) were identified to be potential markers for Zn status with high accuracy (area under the curve [AUC], >0.8). Collectively, this study identified significant changes in gut microbial composition and its metabolite concentration in altered Zn-fed mice and the relevant microbial markers for Zn status. IMPORTANCE Zn insufficiency is an essential health problem in developing countries. To prevent the occurrence of zinc deficit, zinc fortification and supplementation are widely used. However, in developed countries, the amounts of Zn consumed often exceed the tolerable upper intake limit. Our results demonstrated that dietary Zn is an essential mediator of microbial community structure and that both Zn deficiency and Zn overdose can generate a dysbiosis in the gut microbiota. Moreover, specific microbial biomarkers of Zn status were identified and correlated with serum Zn level. Our study found that a short-term low-Zn diet (0 mg/kg) and a long-term high-zinc diet (150 mg/kg) had obvious negative effects in a mouse model. Thus, these results indicate that the provision and duration of supplemental Zn should be approached with caution.