Molecular characterization and functional analysis of a specific secreted protein from highly virulent defoliating Verticillium dahliae
Molecular characterization and functional analysis of a specific secreted protein from highly virulent defoliating Verticillium dahliae
复制标题
高毒力落叶黄萎病特定分泌蛋白的分子表征和功能分析。
DOI：
10.1016/j.gene.2013.06.089
复制
发表时间：
2013-10-01
期刊：
影响因子：
3.5
通讯作者：
Dai, Xiao-Feng
中科院分区：
文献类型：
--
作者：
Liu, Shao-Yan;Chen, Jie-Yin;Dai, Xiao-Feng
黄萎病菌Kleb.是一种植物病原真菌，在数百种双子叶植物中引起枯萎病。以往的研究表明，分泌体在大丽弧菌的致病过程中起着重要作用。本研究克隆了大丽叶病毒高毒力菌株VDG1的分泌蛋白基因(VdSSP1)，通过信号肽活性测定，确定该基因为分泌型蛋白。VDG1中的VdSSP1缺失突变体显著降低了菌株的致病力，并且在以果胶和淀粉为碳源的条件下，真菌的生长受到抑制。补充VdSSP1缺失菌株或缺乏VdSSP1的低毒非落叶菌株VDG2，可恢复致病力和碳素利用。这表明VdSSP1的毒力作用与植物细胞壁的降解有关。综上所述，我们的研究结果表明，VdSSP1是一种分泌型蛋白，参与了强毒落叶大丽叶病毒的致病过程。(C)2013爱思唯尔B.V.保留所有权利。
Verticillium dahliae Kleb. is a phytopathogenic fungus that causes wilt diseases in hundreds of dicotyledonous plant species. Previous research has demonstrated that the secretome plays an important role in the pathogenicity of V. dahliae. In this study, the specific secreted protein gene (VdSSP1) in highly virulent defoliating V. dahliae strain VDG1 was cloned, and considered to be a secreted protein by signal peptide activity assay. VdSSP1 deletion mutants in VDG1 significantly compromised virulence, and the fungal growth decreased in media with pectin and starch as carbon sources. Pathogenicity and carbon utilization were restored upon complementation of the VdSSP1 deletion strains or low virulence non-defoliating strain VDG2, which lacks VdSSP1. It is indicated that the virulence role of VdSSP1 is associated with plant cell wall degradation. In conclusion, our data suggested that VdSSP1 is a secreted protein that is engaged in the pathogenicity of the highly virulent defoliating V. dahliae. (C) 2013 Elsevier B.V. All rights reserved.