Point-of-Care device for quantification of zinc in serum.
Point-of-Care device for quantification of zinc in serum.
复制标题
用于定量血清中锌的即时护理装置。
DOI：
10.1109/embc.2013.6610464
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Beyette,FredR
中科院分区：
文献类型：
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作者：
Zerhusen,Benjamin;deSilva,Geethanga;Pei,Xing;Papautsky,Ian;Beyette,FredR
在美国，每年有42，000例病例报告，死亡率约为10%，[1]儿科感染性休克是一个主要的健康问题，通常难以有效治疗。几项研究表明，经历小儿感染性休克的儿童通常血清锌（Zn）水平极低，这表明补充锌是一种有效的治疗策略。然而，为了保护患者的安全和健康，在补充期间监测血清Zn浓度是极其重要的，以确保水平保持在生理标准或接近生理标准，从而最大限度地减少过量补充的重金属毒性风险。目前用于定量血清中Zn的方法通常需要将血清样品发送到外部实验室设施，导致周转时间从数小时到几天不等。因此，在临床环境中通常不可能及时监测血清中的锌水平，这最终限制了使用锌补充剂作为治疗干预的能力。本文报道了一种用于快速电化学测量血清中锌的床旁装置的开发。该装置以三电极传感器为中心，该传感器使用阳极溶出伏安法（ASV）来感测Zn水平。使用读取器读取Cu基传感器，该读取器是使用市售嵌入式系统部件结合定制模拟电路开发的，能够在大约6分钟内产生定量结果。
With 42,000 cases reported annually in the United States and an approximate 10% mortality rate, [1] pediatric septic shock is a major health problem that is often difficult to treat effectively. Several studies have shown that children experiencing pediatric septic shock often have critically low levels of serum zinc (Zn), suggesting supplementation of Zn to be an effective therapeutic strategy. However, to protect the safety and well-being of the patient, it is extremely important to monitor blood serum concentration of Zn during supplementation in order to ensure that levels remain at or near the physiological norm and thus minimize the risk of heavy metal toxicity from over supplementation. Current methods for quantifying Zn in serum typically require sending serum samples to external laboratory facilities resulting in turnaround times ranging from hours to a few days. Therefore, timely monitoring of Zn levels in serum is often not possible in the clinical setting which ultimately limits the ability to use Zn supplementation as a therapeutic intervention. This paper reports on the development of a Point-of-Care device for rapid electrochemical measurement of Zn in serum. The device is centered on a three electrode sensor which uses Anodic stripping voltammetry (ASV) for sensing Zn levels. The Cu based sensor is read using a reader that has been developed using commercially available embedded system parts in combination with custom analog circuitry that is able to produce quantification results in approximately 6 minutes.