Calcium channels triggering transmitter release in the rat medial superior olive
Calcium channels triggering transmitter release in the rat medial superior olive
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DOI：
10.1016/s0378-5955(01)00378-1
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发表时间：
2001-12-01
期刊：
影响因子：
2.8
通讯作者：
Forsythe, ID
中科院分区：
文献类型：
--
作者：
Barnes-Davies, M;Owens, S;Forsythe, ID
我们使用全细胞电压钳记录大鼠听性脑干脑片的神经元，研究参与触发突触谷氨酸和甘氨酸释放的内侧上级橄榄核的钙通道类型。前腹侧耳蜗（aVCN）丛状神经元突触的谷氨酸释放不涉及L型Ca 2+通道（α（1C-D）; Ca（V）1.2-1.3），但由N型（α（1B）; Ca（V）2.2）和P/Q型Ca 2+通道（α（1A）; Ca（V）2.1）介导，具有相似的功效。斜方体内侧核（MNTB）突触的甘氨酸释放主要由P/Q型Ca ~（2+）通道介导，但N型Ca ~（2+）通道也有重要贡献。联合应用P/Q-和N-型Ca ~（2+）通道毒素，ω-agatoxin IVA和ω-conotoxin GVIA，留下了非常小的抑制性和兴奋性突触后电流的残余，可能反映了R-型Ca ~（2+）通道的最小贡献（α（1 E）; Ca（V）2.3）对递质释放的影响。（alpha（1G-I）; Ca（V）3.1-3.3）和L型通道，但表达更高比例的P/Q型Ca 2+通道。(C)2001 Elsevier Science B. V.保留所有权利。
We used whole cell voltage clamp recordings from neurones in rat auditory brainstem slices to study the Ca2+ channel types involved in triggering synaptic glutamate and glycine release in the medial superior olivary nucleus. Glutamate release from the anterior ventral cochlear (aVCN) bushy neurone synapse did not involve L-type Ca2+ channels (alpha (1C-D); Ca(V)1.2-1.3), but was mediated with similar efficacies by both N-type (alpha (1B); Ca(V)2.2) and the P/Q-type Ca2+ channels (alpha (1A); Ca(V)2.1). Glycine release from the medial nucleus of the trapezoid body (MNTB) synapse was mediated predominantly by P/Q-type Ca2+ channels, but with a significant contribution from N-type Ca2+ Channels. Combined application of the P/Q- and N-type Ca2+ channel toxins, omega -agatoxin IVA and omega -conotoxin GVIA, left a very small remnant of both the inhibitory and excitatory postsynaptic currents, probably reflecting a minimal contribution of R-type Ca2+ channels (alpha (1E); Ca(V)2.3) to transmitter release, In contrast with aVCN bushy neurones, MNTB somata lacked both T-(alpha (1G-I); Ca(V)3.1-3.3) and L-type channels, but expressed a higher proportion of P/Q-type Ca2+ channels. (C) 2001 Elsevier Science B.V. All rights reserved.