Pool Size of Electrons That Can Be Donated to P700+ As Determined in Intact Leaves: Donation to P700+ from Stromal Components Via the Intersystem Chain
Pool Size of Electrons That Can Be Donated to P700+ As Determined in Intact Leaves: Donation to P700+ from Stromal Components Via the Intersystem Chain
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DOI：
10.1093/oxfordjournals.pcp.a078343
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发表时间：
1992-10
影响因子：
4.9
通讯作者：
K. Asada;U. Heber;U. Schreiber
中科院分区：
文献类型：
--
作者：
K. Asada;U. Heber;U. Schreiber
描述了一种估算完整叶片光化光照后P700+电子功能池大小的方法。测定了远红光照射后P700+的稳态水平与远红光照射后50 ms多次周转光（mt区）和光化光照射后P700+的氧化曲线之间的互补面积。自从MT-area代表池大小的电子系统链,职能部门的比例MT-area允许我们估计pooi大小的电子存储在光化照明,可以捐赠给P700 +职能部门的比例MT-area决心几个C3plants完整的叶子,它被发现的两个或三个增加到一个值的增加光化光的强度,进一步增加了乏氧生活。据估计，在有氧条件下，从基质中捐赠给P700+的电子池大小在每个P700 12到28个电子之间。这些结果表明，在c3植物叶绿体中，基质组分通过系统间链向P700+提供电子。
A method for estimation of the functional pool size of electrons that can be donated to P700+after the illumination of intact leaves with actinic light is described. The complementary areas between the stationary level of P700+attained by irradiation with far-red light and the oxidation curves of P700 by far-red light after a 50-ms multiple-turnover light (MT-area), and after the illumination of actinic light (AL-area) were determined. Since the MT-area represents the pool size of electrons in the intersystem chain, the ratio of the AL-area to the MT-area allows us to estimate the pooi size of electrons stored during actinic illumination that can be donated to P700+The ratio of the AL-area to the MT-area was determined for intact leaves of several C3plants, and it was found to increase to a value of two or three with increases in the intensity of actinic light, and it was further increased by anaerobiosis. The pool size of electrons that can be donated to P700+from the stroma was estimated to be in the range of 12 and 28 electrons per P700 under aerobic conditions. These results indicate that stromal components donate electrons to P700+through the intersystem chain in chloroplasts of C3plants.