Water Stress and Protein Synthesis: V. Protein Synthesis, Protein Stability, and Membrane Permeability in a Drought-sensitive and a Drought-tolerant Moss.
Water Stress and Protein Synthesis: V. Protein Synthesis, Protein Stability, and Membrane Permeability in a Drought-sensitive and a Drought-tolerant Moss.
复制标题
水胁迫和蛋白质合成：V. 干旱敏感和耐旱苔藓中的蛋白质合成、蛋白质稳定性和膜渗透性。
作者：
R. Dhindsa;J. Bewley
研究了水分胁迫和干燥对耐旱苔藓 Tortulauralis 和干旱敏感苔藓 Hygrohypnum luridum 蛋白质合成的影响。在任何特定水平的稳态水分胁迫下，H. luridum 中蛋白质合成的抑制均大于 T. mudralis。通过双标记比技术测定，水胁迫引起的蛋白质合成模式的变化在 T. 农村中较小，但在 H. luridum 中主要。发现两种苔藓的蛋白质在干燥和随后的补水过程中都是稳定的。在干燥苔藓的再水化过程中观察到膜渗透性的变化，如氨基酸渗漏所表明的那样，并且膜渗透性的变化取决于干燥速率。在T.乡村中，泄漏量较小且可逆，但在H.luridum中泄漏量较大且不可逆。尽管 H. luridum 未能从完全干燥中恢复（鲜重损失 80%），但在记录到鲜重最大损失 55% 的条件下，它能够从稳态应激中完全恢复。
The effects have been studied of water stress and desiccation on protein synthesis in the drought-tolerant moss Tortula ruralis and the drought-sensitive moss Hygrohypnum luridum. At any particular level of steady state water stress, the inhibition of protein synthesis was greater in H. luridum than in T. ruralis. Water stress-induced changes in the pattern of protein synthesis, as determined by the double label ratio technique, were minor in T. ruralis, but major in H. luridum. Proteins of both mosses were found to be stable during desiccation and subsequent rehydration. Changes in membrane permeability, as indicated by the leakage of amino acid, were observed during rehydration of desiccated moss and were dependent on the rate of desiccation. The leakage was small and reversible in T. ruralis but large and irreversible in H. luridum. Although H. luridum failed to recover from complete desiccation (80% loss in fresh weight), it was able to recover fully from steady state stress under conditions where a maximum loss of 55% in fresh weight was recorded.