P2X2 and P2X5 Receptors Mediate Bladder Hyperesthesia in ICC in Female Overactive Bladder
P2X2 and P2X5 Receptors Mediate Bladder Hyperesthesia in ICC in Female Overactive Bladder
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DOI：
10.1007/s12013-014-0471-x
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发表时间：
2015-06-01
影响因子：
2.6
通讯作者：
Li, Weibing
中科院分区：
文献类型：
--
作者：
Meng, Mingsen;Zheng, Ji;Li, Weibing
本研究旨在探讨P2X(2)和P2X(5)作为膀胱感觉传入重要分子在女性膀胱过度活动症(OAB)膀胱感觉过敏患者中的作用。选取2011年9月—2012年12月第三军医大学附属西南医院收治的68例OAB患者纳入实验组（OAB组），同时纳入30例健康志愿者作为对照组。记录排尿日记和尿动力学结果，并采用免疫组化方法检测女性OAB患者和健康志愿者膀胱组织Caja间质细胞（ICC）P2X（2）和P2X（5）受体，初步探讨P2X（2）和P2X（5）在膀胱感觉过敏中的作用。尿动力学检查对OAB的诊断和鉴别诊断具有重要的诊断价值。 OAB组膀胱ICC中P2X(2)受体显着上调。阻断P2X(2)受体可显着抑制膀胱肌条的收缩，降低膀胱压力和盆腔神经放电。 PET和尿动力学研究显示OAB患者脑桥PAG区排尿欲感较对照组明显增强。 ICC中P2X(2)的上调是引起OAB患者膀胱感觉过敏的重要因素。 PET和尿动力学研究表明膀胱源性神经冲动是OAB的重要病因。本研究为OAB患者膀胱感觉过敏ICC中P2X(2)受体的研究提供依据。
This study was set to explore the role of P2X(2) and P2X(5) as the important molecules in sensory afferent of bladder in female overactive bladder (OAB) patients with the bladder hyperesthesia. Sixty-eight OAB patients admitted in Southwest Hospital affiliated to the Third Military Medical University during September, 2011-December, 2012 were selected and included in the experimental group (OAB group) and 30 healthy volunteers during the same period were included as the control group. We recorded voiding diary and urodynamic results, and immunohistochemistry analysis was used to detect P2X(2) and P2X(5) receptor in interstitial cell of Caja (ICC) in bladder tissue of female OAB patients and healthy volunteers, to tentatively explore the effect of P2X(2) and P2X(5) in bladder hyperesthesia. Urodynamic study has important diagnostic value in the diagnosis and differential diagnosis of OAB. P2X(2) receptor was significantly up-regulated in bladder ICC in OAB group. The blockage of P2X(2) receptor could significantly inhibit the contraction of bladder muscle strips, decrease the bladder pressure and the electric discharge of pelvic nerve. PET and urodynamic study showed that micturition desire sense in PAG area of pons in OAB patients was significantly increased compared with the control group. The up-regulation of P2X(2) in ICC is an important factor to cause bladder hyperesthesia in OAB patients. PET and urodynamic study indicate that the bladder-originated nervous impulses are important cause of OAB. This study provides a basis for the study of P2X(2) receptor in ICC in bladder hyperesthesia of OAB patients.