The white Gene of Drosophila melanogaster Encodes a Protein with a Role in Courtship Behavior
The white Gene of Drosophila melanogaster Encodes a Protein with a Role in Courtship Behavior
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DOI：
10.1080/01677060802309629
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发表时间：
2008-01-01
影响因子：
1.9
通讯作者：
Lloyd, Vett
中科院分区：
文献类型：
--
作者：
Anaka, Matthew;MacDonald, C. Danielle;Lloyd, Vett
黑腹果蝇（Drosophila melanogaster）的白色基因已被广泛研究，但其异位过表达是如何诱导雄性雄性求偶的仍不清楚。为了研究这种行为的细胞基础，我们研究了几类突变体的性行为。我们发现雄雄之间的求偶不仅在过度表达白色基因的果蝇中可见，而且在白色蛋白定位错误的突变体中也可见。这一发现证实了白色转运蛋白在正常表达的细胞中的错误定位会产生雄性雄性的求偶行为；求偶行为并不是身体其他部位异常生理变化的间接结果。在一些单胺代谢改变和学习记忆缺陷的突变体中也可以看到雄性-雄性求偶，但可以通过其强度和运动活动的降低与怀特错定位产生的求偶区别。过表达白色的双突变体和5 -羟色胺能神经元基因突变表明，白色基因错误定位产生的雄性-雄性求爱可能不完全由5 -羟色胺能神经元介导。我们还发现，在白色突变体中，以及在白色结合伴侣棕色和猩红基因突变的个体中，嗅觉学习能力下降。最后，在培养的果蝇和哺乳动物细胞中，在内体室中发现了白色转运蛋白。这里发现的与雄性雄性求爱有关的额外基因增加了研究果蝇性行为的突变库。
The white gene of Drosophila melanogaster has been extensively studied, yet it is still not understood how its ectopic overexpression induces male-male courtship. To investigate the cellular basis of this behavior, we examined the sexual behavior of several classes of mutants. We rind that male-male courtship is seen not only in flies over-expressing the white gene, but also in mutants expected to have mislocalized White protein. This finding confirms that mislocalizing White transporter in the cells in which it is normally expressed will produce male-male courtship behaviors; the courtship behavior is not an indirect consequence of aberrant physiological changes elsewhere in the body. Male-male courtship is also seen in some mutants with altered monoamine metabolism and deficits in learning and memory, but can be distinguished from that produced by White mislocalization by its reduced intensity and locomotor activity. Double mutants overexpressing white and with mutations in genes for serotonergic neurons suggest that male-male courtship produced by mislocalizing White may not be mediated exclusively by serotonergic neurons. We also find decreased olfactory learning in white mutants and in individuals with mutations in the genes for White's binding partners, brown and scarlet. Finally, in cultured Drosophila and mammalian cells, the White transporter is found in the endosomal compartment. The additional genes identified here as being involved in male-male courtship increase the repertoire of mutations available to study sexual behavior in Drosophila.