Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model
Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model
复制标题
DOI：
10.1111/j.1462-5822.2007.01080.x
复制
发表时间：
2008-03-01
影响因子：
3.4
通讯作者：
Cosson, Pierre
中科院分区：
文献类型：
--
作者：
Alibaud, Laeticia;Koehler, Thilo;Cosson, Pierre
人类病原体铜绿假单胞菌以前已被证明使用类似的毒力因子时，感染哺乳动物宿主或网柄阿米巴。在这里，我们随机对铜绿假单胞菌的临床分离株进行突变，并鉴定出对盘基网柄菌毒力减弱的突变体。这些突变菌株在感染果蝇和小鼠时也表现出毒力的强烈下降，证实铜绿假单胞菌利用相似的毒力特征来对抗这些非常不同的宿主。这些细菌突变体的进一步表征表明，TrpD是重要的诱导的群体感应电路，而PchH和PchI参与诱导的III型分泌系统。这些结果证明了网骨藻宿主模型在鉴定和分析铜绿假单胞菌中新的毒力基因方面的有用性和相关性。
The human pathogen Pseudomonas aeruginosa has been shown previously to use similar virulence factors when infecting mammalian hosts or Dictyostelium amoebae. Here we randomly mutagenized a clinical isolate of P. aeruginosa, and identified mutants with attenuated virulence towards Dictyostelium. These mutant strains also exhibited a strong decrease in virulence when infecting Drosophila and mice, confirming that P. aeruginosa makes use of similar virulence traits to confront these very different hosts. Further characterization of these bacterial mutants showed that TrpD is important for the induction of the quorum-sensing circuit, while PchH and PchI are involved in the induction of the type III secretion system. These results demonstrate the usefulness and the relevance of the Dictyostelium host model to identify and analyse new virulence genes in P. aeruginosa.