One-step purification of 3,4-dihydroxyphenyllactic acid, salvianolic acid B, and protocatechualdehyde from Salvia miltiorrhiza bungle by isocratic stepwise hydrogen bond adsorption chromatography on cross-linked 12% agarose
One-step purification of 3,4-dihydroxyphenyllactic acid, salvianolic acid B, and protocatechualdehyde from Salvia miltiorrhiza bungle by isocratic stepwise hydrogen bond adsorption chromatography on cross-linked 12% agarose
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DOI：
10.1093/chromsci/46.2.165
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发表时间：
2008-02-01
影响因子：
1.3
通讯作者：
Janson, J. -C.
中科院分区：
文献类型：
--
作者：
Gu, M.;Su, Z. -G.;Janson, J. -C.
Three major active components of the traditional Chinese medicinal herbSalvia miltiorrhizaBunge, 3,4-dihydroxyphenyllactic acid, salvianolic acid B, and protocatechualdehyde, are separated and purified from a crude water extract in one step by isocratic hydrogen bond adsorption chromatography on cross-linked 12% agarose (Superose 12 HR 10/30). Separation is achieved by stepwise elution with mobile phases composed of mixtures of ethanol and acetic acid: 0–50 mL, 5% ethanol, 5% acetic acid; 50–100 mL, 20% ethanol, 20% acetic acid; and 100–200 mL, 30% ethanol, 30% acetic acid. The 3,4- dihydroxyphenyllactic acid is obtained with a purity of 97.3% and with a recovery of 88.1%. The corresponding figures for protocatechualdehyde are a purity of 99.4% with a recovery of 90.7%, and for salvianolic acid B a purity of 90.4% with a recovery of 50.3%, respectively. At a sample load of 40 mg crude extract dissolved in 0.5 mL mobile phase (corresponding to a load of 1.6 mg/mL gel), a 3,4-dihydroxyphenyllactic acid purity of approximately 94% with a recovery of 80.2% is obtained.
