Molecular deformation and free-solution electrophoresis of DNA-uncharged polymer conjugates at high field strengths: theoretical predictions. Part 1: hydrodynamic segregation.
Molecular deformation and free-solution electrophoresis of DNA-uncharged polymer conjugates at high field strengths: theoretical predictions. Part 1: hydrodynamic segregation.
复制标题
高场强下 DNA 不带电聚合物缀合物的分子变形和自由溶液电泳：理论预测。
DOI：
10.1002/elps.200600590
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发表时间：
2007
期刊：
影响因子：
2.9
通讯作者：
Slater,GaryW
中科院分区：
文献类型：
--
作者：
McCormick,LauretteC;Slater,GaryW
Recent advancements in DNA sequencing by end‐labeled free‐solution electrophoresis (ELFSE) show the promise of this novel technique which overcomes the need for a gel by using a label (or drag‐tag) to render the free solution mobility of the DNA size‐dependent. It is the attachment of an uncharged drag‐tag molecule of a set size to various lengths of DNA in the sample that selectively slows down smaller DNA chains which have less force to pull the drag‐tag than larger DNA. So far, only globally random coil conformations have been associated with ELFSE,i.e., the DNA and the label together form a single, undeformed hydrodynamic unit. This paper investigates the conditions under which the DNA and label will segregate into two hydrodynamically distinct units, based on a theoretical approach developed for the electrophoresis of polyampholytes. Optimal experimental conditions tailored to the available label sizes and voltages are predicted along with insight into ideal label architecture.
