Arabidopsis AtPRP17 functions in embryo development by regulating embryonic patterning
Arabidopsis AtPRP17 functions in embryo development by regulating embryonic patterning
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DOI：
10.1007/s00425-021-03702-4
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发表时间：
2021-08
期刊：
影响因子：
4.3
通讯作者：
D. Shao;Y. Wei;Z. Yu;Xinren Dai;Xin-Qi Gao
中科院分区：
文献类型：
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作者：
D. Shao;Y. Wei;Z. Yu;Xinren Dai;Xin-Qi Gao
主要结论拟南芥AtPRP 17是酵母剪接因子基因PRP 17的同源物，在长角果和胚胎中表达，并通过调控胚胎发育来发挥作用，酵母剪接因子PRP 17/CDC 40通过参与细胞周期调控而对细胞生长起重要作用，拟南芥基因组编码一个类似物AtPRP 17，但其在拟南芥发育中的功能尚不清楚。本研究表明，AtPRP 17在角果和胚中高表达，且定位于细胞核。AtPRP 17功能缺失突变导致假成熟角果种子皱缩。进一步分析表明，突变体的缺陷成熟种子是由于子叶皱缩、不等长、下胚轴肿胀缩短或胚根缩短等异常胚造成的。在胚胎发生过程中，突变体胚胎表现出发育延迟和顶端和基部结构域的缺陷，如抑制子叶和紊乱的静止中心细胞和柱。我们的研究结果表明AtPRP 17通过调控胚胎的图案形成而在胚胎发育中起作用。
Main conclusionArabidopsis AtPRP17, a homolog of yeast splicing factor genePRP17, is expressed in siliques and embryos and functions in embryo development via regulating embryonic patterning.AbstractYeast splicing factor PRP17/CDC40 is essential for cell growth through involvement in cell cycle regulation.Arabidopsisgenome encodes a homolog of PRP17, AtPRP17; however, its function inArabidopsisdevelopment is unknown. This study showed thatAtPRP17was highly expressed in siliques and embryos, and the protein was localized in the nucleus. The loss-of-function mutation ofAtPRP17led to shrunken seeds inArabidopsismature siliques. Further analysis revealed that the defective mature seeds of the mutant resulted from abnormal embryos with shriveled cotyledons, unequal cotyledons, swollen and shortened hypocotyls, or shortened radicles. During embryogenesis, mutant embryos showed delayed development and defective patterning of the apical and base domains, such as inhibited cotyledons and disorganized quiescent center cells and columella. Our results suggested that AtPRP17 functions inArabidopsisembryo development via regulating embryonic patterning.