An intronless gene encoding a poly(A) polymerase is specifically expressed in testis
An intronless gene encoding a poly(A) polymerase is specifically expressed in testis
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DOI：
10.1016/s0014-5793(00)02367-x
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发表时间：
2000-12-29
期刊：
影响因子：
3.5
通讯作者：
Chung, JH
中科院分区：
文献类型：
--
作者：
Lee, YJ;Lee, Y;Chung, JH
以前的工作表明，一个单一的前体mRNA可以通过选择性剪接或选择性聚腺苷酸化产生多种形式的哺乳动物poly（A）聚合酶mRNA。在这项研究中，一个cDNA编码睾丸特异性聚（A）聚合酶的分离。2.5周龄小鼠睾丸组织中Papt基因的转录水平明显低于一般的poly（A）聚合酶基因Pnp。然而，在4周龄小鼠的睾丸中，Pap与Pap的转录比例被逆转。瞬时表达分析表明，GFP-Papt融合蛋白在HeLa细胞的细胞核和细胞质中均有表达。这些结果表明，Papt参与精子发生过程中表达的转录本的多聚腺苷酸化。(C)2000年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
Previous work demonstrated that a single pre-mRNA could generate multiple forms of mammalian poly(A) polymerase mRNAs by alternative splicing or alternative polyadenylation. A cDNA encoding a testis-specific poly(A) polymerase was isolated in this study. The transcription level of Papt in testis of a 2,weeks old mouse was much lower than that of the general poly(A) polymerase gene, Pnp. However, the transcription ratio of Papt to Pap was reversed in testis of a 4 weeks old mouse. Transient expression analysis showed that GFP-Papt fusion protein is present both in the nucleus and cytoplasm of HeLa cells. These results suggest that Papt is involved in polyadenylation of transcripts expressed during spermatogenesis. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.