The diurnal logic of the expression of the chloroplast genome in Chlamydomonas reinhardtii.
The diurnal logic of the expression of the chloroplast genome in Chlamydomonas reinhardtii.
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DOI：
10.1371/journal.pone.0108760
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bock R
中科院分区：
文献类型：
--
作者：
Idoine AD;Boulouis A;Rupprecht J;Bock R
叶绿体来源于蓝细菌，并保留了细菌型基因组和基因表达机制。叶绿体基因组编码类囊体膜中光合机构的许多核心成分。为了避免光氧化损伤和有害的活性氧（ROS）的不完全组装的类囊体蛋白复合物的生产，叶绿体基因的表达必须严格调控和协调的基因在细胞核中的表达。在全基因组水平上，叶绿体基因表达受内部节律和外部信号的调控，这方面的研究知之甚少。为了获得昼夜条件下单细胞模型莱茵衣藻中细胞器转录物水平的全面图片，开发了qRT-PCR平台，并用于定量在高度受控的12 h光照/12 h黑暗循环中生长的细胞中的68个叶绿体、21个线粒体以及71个核转录物。有趣的是，在黄昏的预期中，叶绿体转录基因的转录本首先达到峰值水平，然后是翻译基因的转录本，最后是光合作用基因的转录本。这种模式完全符合细胞从夜间“醒来”的理论要求。在核转录本中观察到类似的趋势。这些结果表明叶绿体基因组的表达中有一个惊人的内在逻辑，并且叶绿体基因的调控具有以前未被认识到的复杂性。
Chloroplasts are derived from cyanobacteria and have retained a bacterial-type genome and gene expression machinery. The chloroplast genome encodes many of the core components of the photosynthetic apparatus in the thylakoid membranes. To avoid photooxidative damage and production of harmful reactive oxygen species (ROS) by incompletely assembled thylakoid protein complexes, chloroplast gene expression must be tightly regulated and co-ordinated with gene expression in the nucleus. Little is known about the control of chloroplast gene expression at the genome-wide level in response to internal rhythms and external cues. To obtain a comprehensive picture of organelle transcript levels in the unicellular model alga Chlamydomonas reinhardtii in diurnal conditions, a qRT-PCR platform was developed and used to quantify 68 chloroplast, 21 mitochondrial as well as 71 nuclear transcripts in cells grown in highly controlled 12 h light/12 h dark cycles. Interestingly, in anticipation of dusk, chloroplast transcripts from genes involved in transcription reached peak levels first, followed by transcripts from genes involved in translation, and finally photosynthesis gene transcripts. This pattern matches perfectly the theoretical demands of a cell “waking up” from the night. A similar trend was observed in the nuclear transcripts. These results suggest a striking internal logic in the expression of the chloroplast genome and a previously unappreciated complexity in the regulation of chloroplast genes.
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DOI：
10.1073/pnas.91.23.10819
发表时间：
1994-11-08
影响因子：
11.1
作者：
KLEIN, U;SALVADOR, ML;BOGORAD, L
通讯作者：
BOGORAD, L
DOI：
10.1073/pnas.89.8.3453
发表时间：
1992-04-15
影响因子：
11.1
作者：
KLEIN, U;DECAMP, JD;BOGORAD, L
通讯作者：
BOGORAD, L
影响因子：
5.3
作者：
Matsuo, T;Onai, K;Ishiura, M
通讯作者：
Ishiura, M
影响因子：
7.2
作者：
Eberhard, S;Drapier, D;Wollman, FA
通讯作者：
Wollman, FA
影响因子：
11.6
作者：
Ramundo, Silvia;Rahire, Michele;Rochaix, Jean-David
通讯作者：
Rochaix, Jean-David