DISCRETE PHASE-TRANSITION OF GIANT DNA DYNAMICS OF GLOBULE FORMATION FROM A SINGLE MOLECULAR CHAIN
DISCRETE PHASE-TRANSITION OF GIANT DNA DYNAMICS OF GLOBULE FORMATION FROM A SINGLE MOLECULAR CHAIN
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DOI：
10.1016/0167-2789(95)00020-5
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发表时间：
1995-06-15
期刊：
影响因子：
4
通讯作者：
MATSUZAWA, Y
中科院分区：
文献类型：
--
作者：
YOSHIKAWA, K;MATSUZAWA, Y
我们通过荧光显微镜直接观察单个DNA的构象，发现双链DNA分子的单链经历了一级相变。很明显，一条DNA链以离散的方式在扩张的线圈和收缩的小球之间改变其高阶结构。处于线圈状态的单条DNA链所占据的有效体积约为。比球状状态大10(4)倍。通过使用荧光显微镜测量单个 DNA 分子来分析小球形成或成核和生长的动态过程。我们展示了 DNA 线圈的表观轮廓长度以恒定速度减小，同时伴随着球状结构的生长。
We found that a single chain of double strand DNA molecule undergoes a first-order phase transition by the direct observation of the conformation in individual DNA with fluorescence microscopy. It has become clear that a single DNA chain changes its higher order structure between expanded coil and shrunken globule in a discrete manner. The effective volume occupied by a single DNA chain in the coil state is ca. 10(4) times greater than that in the globule state. The dynamic process of the globule formation, or nucleation and growth, is analyzed by measuring individual DNA molecules with fluorescence microscopy. We show chat the apparent contour length of the DNA coil decreases with a constant speed, accompanied by the growth of the globular structure.