Sequence analysis and expression of the two genes for elongation factor 1 alpha from the dimorphic yeast Candida albicans.
Sequence analysis and expression of the two genes for elongation factor 1 alpha from the dimorphic yeast Candida albicans.
复制标题
二态性酵母白色念珠菌延伸因子 1 α 的两个基因的序列分析和表达。
DOI：
10.1128/jb.172.4.2036-2045.1990
复制
发表时间：
1990
影响因子：
3.2
通讯作者：
Sypherd,PS
中科院分区：
文献类型：
--
作者：
Sundstrom,P;Smith,D;Sypherd,PS
利用从总状毛霉中提取的TEF1探针，克隆了2个编码蛋白合成因子伸长因子1 α (EF-1 α)的白色念珠菌基因，筛选了白色念珠菌基因组DNA文库。两个克隆的序列分析表明，两个基因编码区两侧的DNA区域不同源，验证了白色念珠菌中EF-1 α的两个基因TEF1和TEF2的存在。TEF1和TEF2编码区仅相差5个核苷酸，编码相同的EF-1 α蛋白，共458个氨基酸。这两个基因在体内转录成mRNA，通过寡核苷酸探针杂交，分别特异性结合TEF1和TEF2的3'非翻译区，在Northern （RNA）印迹分析中与白色念珠菌总RNA结合。白色念珠菌预测的EF-1 α蛋白与酿酒酵母EF-1 α的相似性大于与总状芽孢杆菌EF-1 α的相似性。此外，白色假丝酵母菌EF-1 α蛋白的密码子偏向性、启动子和终止子信号与酿酒酵母EF-1 α具有显著的相似性。综上所述，这些结果表明白色念珠菌与酿酒子囊菌的亲缘关系比与总形颧菌的亲缘关系更密切。
Two Candida albicans genes that encode the protein synthesis factor elongation factor 1 alpha (EF-1 alpha) were cloned by using a heterologous TEF1 probe from Mucor racemosus to screen libraries of C. albicans genomic DNA. Sequence analysis of the two clones showed that regions of DNA flanking the coding regions of the two genes were not homologous, verifying the presence of two genes, called TEF1 and TEF2, for EF-1 alpha in C. albicans. The coding regions of TEF1 and TEF2 differed by only five nucleotides and encoded identical EF-1 alpha proteins of 458 amino acids. Both genes were transcribed into mRNA in vivo, as shown by hybridization of oligonucleotide probes, which bound specifically to the 3' nontranslated regions of TEF1 and TEF2, respectively, to C. albicans total RNA in Northern (RNA) blot analysis. The predicted EF-1 alpha protein of C. albicans was more similar to Saccharomyces cerevisiae EF-1 alpha than to M. racemosus EF-1 alpha. Furthermore, codon bias and the promoter and termination signals of the C. albicans EF-1 alpha proteins were remarkably similar to those of S. cerevisiae EF-1 alpha. Taken together, these results suggest that C. albicans is more closely related to the ascomycete S. cerevisiae than to the zygomycete M. racemosus.