Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov.
Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov.
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基因组序列和厌氧木质素降解细菌的描述。十一月。
DOI：
10.1186/s40793-015-0100-3
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发表时间：
2015
影响因子：
--
通讯作者：
DeAngelis KM
中科院分区：
文献类型：
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作者：
Billings AF;Fortney JL;Hazen TC;Simmons B;Davenport KW;Goodwin L;Ivanova N;Kyrpides NC;Mavromatis K;Woyke T;DeAngelis KM
TolumonaslignolyticaBRL 6 - 1Tsp. nov.是该菌的模式菌株。lignolytica sp. nov.，一个被提出的Tolumonas属的新物种。该菌株分离自热带雨林土壤，基于其利用木质素作为唯一碳源的能力。解木质甲苯单胞菌BRL 6 - 1T的细胞是嗜温的、不形成孢子的、革兰氏阴性杆菌，其是氧化酶和过氧化氢酶阴性的。对该分离物的基因组进行测序，并返回7个独特的重叠群，总计3.6Mbp，从而能够表征木质素分解的几种推定途径。特别地，我们发现了参与木质素解聚的细胞外过氧化物酶，以及参与β-芳基醚键断裂的几种酶，这是木质素单体之间最丰富的键。我们还发现了参与阿魏酸代谢的酶的基因，阿魏酸是木质素分解的常见产物。通过表征在厌氧环境中木质素的细菌分解中使用的途径和酶，这项工作应有助于从木质纤维素材料生产生物燃料的有效工程。本文的在线版本（doi：10.1186/s40793 - 015 - 0100 - 3）包含补充材料，可供授权用户使用。
Tolumonas lignolytica BRL6-1T sp. nov. is the type strain of T. lignolytica sp. nov., a proposed novel species of the Tolumonas genus. This strain was isolated from tropical rainforest soils based on its ability to utilize lignin as a sole carbon source. Cells of Tolumonas lignolytica BRL6-1T are mesophilic, non-spore forming, Gram-negative rods that are oxidase and catalase negative. The genome for this isolate was sequenced and returned in seven unique contigs totaling 3.6Mbp, enabling the characterization of several putative pathways for lignin breakdown. Particularly, we found an extracellular peroxidase involved in lignin depolymerization, as well as several enzymes involved in β-aryl ether bond cleavage, which is the most abundant linkage between lignin monomers. We also found genes for enzymes involved in ferulic acid metabolism, which is a common product of lignin breakdown. By characterizing pathways and enzymes employed in the bacterial breakdown of lignin in anaerobic environments, this work should assist in the efficient engineering of biofuel production from lignocellulosic material. The online version of this article (doi:10.1186/s40793-015-0100-3) contains supplementary material, which is available to authorized users.