Uptake of the retrograde fluorescent tracer fast blue from the cerebrospinal fluid of the rat.
Uptake of the retrograde fluorescent tracer fast blue from the cerebrospinal fluid of the rat.
复制标题
从大鼠脑脊液中摄取逆行荧光示踪剂坚蓝。
DOI：
10.1016/0304-3940(85)90584-1
复制
发表时间：
1985
影响因子：
2.5
通讯作者：
Eisenman,LM
中科院分区：
文献类型：
--
作者：
Eisenman,LM
将荧光染料固蓝（FB）注射到蛛网膜下腔导致许多脑干和前脑结构中细胞的逆行标记。这些核包括小脑前核、颅神经核、中缝核、网状结构和产生脑干表面附近的神经束的区域。这些结果提出了一个严重的来源，潜在的错误（假阳性），在逆行追踪研究使用FB和其他荧光染料。更重要的是，它们证明了可能的潜在途径，即穿过轴突膜，或被脑表面的轴突末端摄取，通过这些途径，包括毒素在内的化合物可以经由脑脊液进入中枢神经系统。
Injections of the fluorescent dye Fast blue (FB) into the subarachnoid space result in the retrograde labeling of cells in many brainstem and forebrain structures. These nuclei include precerebellar nuclei, cranial nerve nuclei, raphe nuclei, reticular formation and regions giving rise to tracts traveling near the surface of the brainstem. These results present a serious source of potential error (false positives) in retrograde tracing studies using FB and perhaps other fluorescent dyes. More importantly, they demonstrate possible potential routes, i.e. across the axonal membrane, or uptake by axon terminals at the brain surface, by which compounds, including toxins, can gain entrance into the central nervous system via the cerebrospinal fluid.