IL-17A expression in the adenoid tissue from children with sleep disordered breathing and its association with pneumococcal carriage.
IL-17A expression in the adenoid tissue from children with sleep disordered breathing and its association with pneumococcal carriage.
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DOI：
10.1038/s41598-018-35169-x
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发表时间：
2018-11-13
影响因子：
4.6
通讯作者：
Chiu CH
中科院分区：
文献类型：
--
作者：
Huang CC;Wu PW;Chen CL;Wang CH;Lee TJ;Tsai CN;Chiu CH
扁桃体和腺样体组织肥大（AH）是小儿睡眠呼吸障碍（SDB）最常见的原因，AH可能是由反复暴露于感染性病原体或过敏原引起的。在此，我们评估了SDB患儿腺样体组织中IL-17 A的活性及其与AH和肺炎球菌携带的关系。35名患有SDB并接受腺样体切除术和扁桃体切除术的儿童（年龄3-12岁）入选。术中鼻咽拭子细菌培养和多重PCR检测鼻咽部携带情况，并采集腺样体标本。通过实时PCR和蛋白质印迹法评估IL-17 A和相关细胞因子的表达。mRNA分析显示，在AH的腺样体组织中，IL-17 A水平、IL-17 A：IL-10比率和RAR相关孤儿受体-γt：叉头盒P3比率显著较高，在肺炎球菌携带的腺样体组织中，IL-17 A水平和IL-17 A：IL-10比率也显著较高。此外，肺炎球菌携带者在鼻咽腺样体中更常见于无AH的患者。IL-17 A在AH和肺炎球菌携带患者的腺样体组织中上调。这些结果表明，肺炎球菌携带启动IL-17 A介导的免疫反应，在鼻咽腺样肿，这可能与AH的SDB患者。
Tonsil and adenoid-tissue hypertrophy (AH) is the most common cause of pediatric sleep-disordered breathing (SDB), with AH possibly initiated by repeated exposure to infectious agents or allergens. Here, we evaluated IL-17A activity in adenoid tissue from children with SDB and its association with AH and pneumococcal carriage. Thirty-five children (aged 3–12 years) with SDB and receiving adenoidectomy and tonsillectomy were enrolled. During surgery, nasopharyngeal carriage was determined by bacterial culture and multiplex PCR via nasopharyngeal swab, and adenoid samples were collected. IL-17A and associated cytokine expression was evaluated by real-time PCR and western blotting. The mRNA analysis showed that IL-17A level, IL-17A:IL-10 ratio, and RAR-related orphan receptor-γt:forkhead box P3 ratio were significantly higher in adenoid tissues with AH, as were IL-17A level and IL-17A:IL-10 ratio in adenoid tissues with pneumococcal carriage. Additionally, pneumococcal carriage was more common in nasopharyngeal adenoids from patients without AH than those with AH. IL-17A was upregulated in adenoid tissues from patients with AH and with pneumococcal carriage. These results suggested that pneumococcal carriage initiates an IL-17A-mediated immune response in nasopharyngeal adenoids, which might be associated with AH in patients with SDB.
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