Molecular mechanisms of eukaryotic origin initiation, replication fork progression, and chromatin maintenance.
Molecular mechanisms of eukaryotic origin initiation, replication fork progression, and chromatin maintenance.
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真核起源启动、复制分叉进程和染色质维持的分子机制。
DOI：
10.1042/bcj20200065
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发表时间：
2020-09-30
期刊：
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Yuan Z;Li H
真核生物DNA复制是一个高度动态和严格调控的过程。复制涉及数十种复制蛋白，包括启动子ORC和Cdc6、复制CMG解旋酶、DNA聚合酶α-引物酶、前导链DNA聚合酶ε和滞后链DNA聚合酶δ。这些蛋白质以空间和时间控制的方式共同作用，从亲本DNA模板合成新的DNA。在DNA复制过程中，印在DNA和组蛋白上的表观遗传信息也被复制到子DNA中，以维持染色质状态。DNA甲基转移酶1主要负责将亲本DNA甲基化模式复制到新生DNA中。编码在组蛋白中的表观遗传信息是通过一个更复杂和不太为人所知的过程传递的，这个过程被称为复制偶对核小体组装。在这里，我们总结了DNA复制起始、复制叉延伸、染色质组装和维持以及相关调控机制的最新结构和生化见解。
Eukaryotic DNA replication is a highly dynamic and tightly regulated process. Replication involves several dozens of replication proteins, including the initiators ORC and Cdc6, replicative CMG helicase, DNA polymerase α-primase, leading-strand DNA polymerase ε, and lagging-strand DNA polymerase δ. These proteins work together in a spatially and temporally controlled manner to synthesize new DNA from the parental DNA templates. During DNA replication, epigenetic information imprinted on DNA and histone proteins is also copied to the daughter DNA to maintain the chromatin status. DNA methyltransferase 1 is primarily responsible for copying the parental DNA methylation pattern into the nascent DNA. Epigenetic information encoded in histones is transferred via a more complex and less well understood process termed replication-couple nucleosome assembly. Here, we summarize the most recent structural and biochemical insights into DNA replication initiation, replication fork elongation, chromatin assembly and maintenance, and related regulation mechanisms.
影响因子：
3
作者：
Clarey, Megan G.;Botchan, Michael;Nogales, Eva
通讯作者：
Nogales, Eva