Suppressor cell activity after concanavalin A treatment of lymphocytes from normal donors
Suppressor cell activity after concanavalin A treatment of lymphocytes from normal donors
复制标题
正常供体淋巴细胞经伴刀豆球蛋白 A 处理后抑制细胞活性
DOI：
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发表时间：
1976
影响因子：
15.3
通讯作者：
R. Good
中科院分区：
文献类型：
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作者：
L. Shou;S. Schwartz;R. Good
用植物凝集素伴刀豆球蛋白 A (Con A) 预处理正常人外周血淋巴细胞，可抑制单向混合白细胞培养中来自健康志愿者供体的未经处理的同种异体淋巴细胞的母细胞转化和 [3H] 胸苷掺入。类似地，经Con A处理的同种异体细胞可抑制对有丝分裂原、某些微生物抗原和同种异体淋巴细胞的反应。 Con A 预处理的自体淋巴细胞也可被诱导表现出抑制活性。这种抗有丝分裂作用的发生没有细胞毒性的证据，并且对新生淋巴细胞反应具有活性，并且不需要预先对所测试的细胞进行致敏。 Con A 预处理的抑制细胞对淋巴细胞对商陆有丝分裂原（一种有效的 B 细胞刺激剂）的反应的抑制，与对其他有丝分裂原（包括植物血凝素和 Con A）的反应的抑制作用并不一致。此外，对淋巴细胞向微生物抗原、结核菌素纯化蛋白衍生物和白色念珠菌提取物转化的抑制也可以通过 Con A 预处理同种异体或自体细胞类似地诱导。细胞。在健康供体的淋巴细胞中诱导自体抑制活性与包括抑制细胞在调节正常免疫反应中的作用的模型相一致。
Pretreatment of normal human peripheral blood lymphocytes with the plant lectin, concanavalin A (Con A), results in inhibition of blast transformation and [3H]thymidine incorporation by untreated allogeneic lymphocytes from healthy volunteers donors in one-way mixed leukocyte culture. Similarly, responses to mitogens, certain microbial antigens, and allogeneic lymphocytes are inhibited by Con A-treated allogeneic cells. Con A pretreated autologous lymphocytes can also be induced to manifest suppressor activities. This antimitotic effect occurs without evidence of cytotoxicity and is active on de novo lymphocyte responses and does not require prior sensitization of the cells being tested. Suppression of the lymphocyte response to pokeweed mitogen, a potent B- cell stimulator, by Con A-pretreated suppressor cells was not as consistent as was inhibition of response to other mitogens, including phytohemagglutinin and Con A. Furthermore, suppression of lymphocyte transformation to the microbial antigens, tuberculin purified protein derivative, and Canadida albicans extracts could be similarly induced by Con A pretreatment of either allogeneic or autologous cells. Induction of autologous suppressor activity in lymphocytes from healthy donors is compatible with a model that includes a role for suppressor cells in the modulation of the normal immune response.