[A detection method for recombinant DNA from genetically modified maize CBH351].
[A detection method for recombinant DNA from genetically modified maize CBH351].
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一种转基因玉米CBH351重组DNA的检测方法
DOI：
10.3358/shokueishi.42.197
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
A. Hino
中科院分区：
文献类型：
--
作者：
T. Matsuoka;H. Kuribara;S. Suefuji;H. Miura;Y. Kusakabe;H. Akiyama;Y. Goda;K. Isshiki;M. Toyoda;A. Hino
设计了一种使用聚合酶链反应 (PCR) 的方法来检测转基因玉米 CBH351，该玉米尚未在日本获得安全用于食品和饲料的授权。我们分析了引入 CBH351 玉米的重组 DNA (r-DNA) 序列，并设计了特异性引物对来扩增包括部分 r-DNA 的片段。使用设计的引物对获得的 PCR 产物对 CBH351 具有特异性，应防止其他玉米和其他主要谷类作物引起的假阳性结果。可以从含有 0.05-0.1% CBH351 玉米的玉米样品中检测到引入 CBH351 的 r-DNA。这种灵敏度理论上相当于多个基因组拷贝的水平，因此该技术是检测 CBH351 玉米的非常有效的方法。
A method using polymerase chain reaction (PCR) was designed for the detection of genetically modified maize CBH351, which has not authorized as safe for use in foods and feeds in Japan yet. We analyzed a recombinant DNA (r-DNA) sequence introduced into CBH351 maize and designed specific primer pairs to amplify a segment including part of the r-DNA. The PCR products obtained by using the designed primer pairs are specific for CBH351 and should prevent false positive results caused by other maizes and other main cereal crops. The r-DNA introduced into CBH351 could be detected from maize samples containing 0.05-0.1% CBH351 maize. This sensitivity is theoretically equivalent to a level of several genome copies and so this technique is a very efficient means to detect CBH351 maize.