cDNA subtraction cloning reveals novel genes whose temporal and spatial expression indicates association with trophoblast invasion
cDNA subtraction cloning reveals novel genes whose temporal and spatial expression indicates association with trophoblast invasion
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DOI：
10.1006/dbio.2000.9705
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发表时间：
2000-06-01
影响因子：
2.7
通讯作者：
Fundele, R
中科院分区：
文献类型：
--
作者：
Hemberger, M;Himmelbauer, H;Fundele, R
滋养层侵袭是大多数哺乳动物发育的关键过程，与肿瘤细胞的侵袭行为有相似之处。在本研究中，在小鼠发育第 8 天的侵入性滋养层和妊娠第 18 天的成熟非侵入性胎盘之间构建了 cDNA 消减文库。差异表达克隆之一 Epcs26 被定位到 X 染色体，显示与任何已知基因没有同源性。它主要表达于壁层内胚层、外胎盘锥的未分化细胞和少数滋养层巨细胞中。另一个被命名为 Epcs50 的基因被定位到 19 号染色体。它与小鼠 Mps1 基因表现出同源性，并且与 Mps1 一样，可能与裂解蛋白穿孔素有较远的关系。在壁层内胚层细胞和次级滋养层巨细胞亚群中检测到高表达。两个序列，Epcs24 和 Epcs68，表现出广泛的开放阅读框，具有半胱氨酸蛋白酶组织蛋白酶 L 的共同特征。表达仅限于滋养层巨细胞的未定义亚群。这两个基因均定位在 13 号染色体上，与组织蛋白酶 L 和 I 非常接近。MPS1 和组织蛋白酶 L 蛋白的已知功能表明相关蛋白 EPCS50、EPCS24 和 EPCS68 参与赋予小鼠滋养层侵袭特性。 (C) 2000 年学术出版社。
Trophoblast invasion is a critical process in development of most mammals that shares similarities with the invasive behavior of tumor cells. In the present investigation, a cDNA subtraction library was constructed between invasive trophoblast at day 8 of murine development and mature noninvasive placenta at day 18 of gestation. One of the differentially expressed clones, Epcs26, was mapped to the X chromosome and revealed no homology to any known gene. It was predominantly expressed in parietal endoderm, undifferentiated cells of the ectoplacental cone, and a few trophoblast giant cells. Another gene, designated Epcs50, was mapped to chromosome 19. It exhibited homologies to the mouse Mps1 gene and, like Mps1, may have a distant relationship to the lytic protein perforin. High expression was detected in parietal endoderm cells and in a subset of secondary trophoblast giant cells. Two sequences, Epcs24 and Epcs68, exhibited an extensive open reading frame that shared the common features of the cysteine proteinase cathepsin L. Expression was confined to an undefined subpopulation of trophoblast giant cells. Both genes were mapped to chromosome 13 in close proximity to cathepsins L and I. The known functions of MPS1 and cathepsin L proteins indicate that the related proteins EPCS50, EPCS24, and EPCS68 participate in conferring invasive properties to the mouse trophoblast. (C) 2000 Academic Press.