Experimental study of osteoinduction using a new material as a carrier for bone morphogenetic protein-2
Experimental study of osteoinduction using a new material as a carrier for bone morphogenetic protein-2
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DOI：
10.1016/j.bjoms.2010.05.010
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发表时间：
2011-06-01
影响因子：
1.8
通讯作者：
Bessho, Kazuhisa
中科院分区：
文献类型：
--
作者：
Koyama, Noriaki;Okubo, Yasunori;Bessho, Kazuhisa
我们评估了有用的人工胶原蛋白作为一种新的载体重组人骨形态发生蛋白-2（rhBMP-2），通过比较它与端肽胶原蛋白，这是来自猪皮，我们以前已经证明是有用的骨诱导。将rhBMP-2 5 μ g与端肽胶原3 mg或人工胶原3 mg一起植入10周龄Wistar大鼠的小腿肌肉中（每组n = 3）。3只大鼠单独给予人工胶原作为对照（n = 3）。植入后第21天进行放射学评价、组织学分析和生化检查。两组软X线片（波长10-0.10 nm）均显示不透明阴影。组织学分析显示，两个实验组中均形成了新骨。在去端肽组中，在植入的胶原的最外边缘处发现软骨内骨化。而人工胶原组植入的胶原骨化较少。生化检测结果显示，两个实验组的碱性磷酸酶活性和钙离子浓度均高于对照组，缺肽组高于人工胶原组。我们的研究结果表明，人工胶原是有用的载体rhBMP-2的设计，以促进新骨的形成。(C)2010年英国口腔颌面外科医师协会。由爱思唯尔有限公司出版。保留所有权利。
We evaluated the usefulness of artificial collagen as a new carrier for recombinant human bone morphogenetic protein-2 (rhBMP-2) by comparing it with that of atelopeptide collagen, which is derived from porcine skin, and which we have previously shown to be useful for the induction of bone. rhBMP-2 5 p,g with either atelopeptide collagen 3 mg or artificial collagen 3 mg was implanted into the calf muscle of 10-week-old Wistar rats (n = 3 in each group). Three rats were given artificial collagen alone and acted as controls (n = 3). Radiographic evaluation, histological analysis, and biochemical examinations were made on day 21 after implantation. Soft radiographs (wavelength 10-0.10 nm) showed opaque shadows in both groups. Histological analysis showed that new bone had formed in both experimental groups. Endochondral ossification was found at the outermost edge of the implanted collagen in the atelopeptide group. However, there was less ossification in the implanted collagen in the artificial collagen group. On biochemical examination, alkaline phosphatase activity and calcium concentrations in both experimental groups were higher than in the control group, and were higher in the atelopeptide group than in the artificial collagen group. Our results suggest that artificial collagen is useful as a carrier for rhBMP-2 designed to promote the formation of new bone. (C) 2010 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.